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dc.contributor.authorSánchez-Cordón, Pedro J.es_ES
dc.contributor.authorJabbar, Tamaraes_ES
dc.contributor.authorChapman, Davees_ES
dc.contributor.authorDixon, Lindaes_ES
dc.contributor.authorMontoya, Maríaes_ES
dc.identifier.citationJ Virol 94 (14) e00350-20 (2020)es_ES
dc.description21 p.-11 fig.es_ES
dc.description.abstractFollowing short immunization protocols, naturally attenuated African swine fever virus (ASFV) isolate OURT88/3 and deletion mutant BeninDeltaMGF have previously been shown to induce high percentage of protection in domestic pigs against challenge with virulent virus. Results obtained in the present study showed that a single intramuscular immunization of domestic pigs with OURT88/3 or BeninDeltaMGF followed by a challenge with virulent Benin 97/1 isolate at day 130 post-immunisation did not trigger the necessary mechanisms to generate immunological memory able to induce long-term protection against disease. All pigs developed acute forms of ASF. IFNgamma producing cells peaked at day 24 post-immunisation, declining thereafter. Surprisingly, levels of T regulatory cells (Tregs) and IL-10 were elevated at the end of the experiment suggesting that regulatory components of the immune system may inhibit effective protection.Importance. Duration of immunity for any vaccine candidate is crucial. In the case of African swine fever virus vaccine candidates, this issue has received little attention. Attenuated viruses have been proven protective following short immunization protocols in which pigs were challenged a few weeks after the first immunization. Here, duration of immunity and immune responses induced over a duration of 130 days were studied during pre-challenge and after challenge of pigs immunised with the naturally attenuated isolate OURT88/3 and an attenuated gene-deleted isolate BeninDeltaMGF. After a single intramuscular immunization of domestic pigs with the OURT88/3 isolate o BeninDeltaMGF virus, animals were not protected against challenge with virulent Benin 97/1 ASFV genotype I isolate at day 130 post-immunization. Levels of T regulatory cells and IL-10 were elevated at the end of the experiment, suggesting that regulatory components of the immune system may inhibit effective protection.es_ES
dc.description.sponsorshipThis study was funded by the UK’s BBSRC grants BBS/E/I/00002014, BBS/E/1/00007031, BBS/E/1/00007034 and BB/L004267/1.es_ES
dc.publisherAmerican Society for Microbiologyes_ES
dc.relation.isversionofPublisher's versiones_ES
dc.subjectAfrican swine fever viruses_ES
dc.subjectAttenuated vaccineses_ES
dc.subjectLong-term immunityes_ES
dc.subjectRegulatory T cellses_ES
dc.subjectInterleukin 10es_ES
dc.titleAbsence of long-term protection in domestic pigs immunized with attenuated African swine fever virus isolate OURT88/3 or BeninΔMFG correlates with increased levels of regulatory T cells and IL-10es_ES
dc.title.alternativeRunning title: Regulatory T cells interfere protection against ASFVes_ES
dc.description.peerreviewedPeer reviewedes_ES
dc.contributor.funderBiotechnology and Biological Sciences Research Council (UK)es_ES
oprm.item.hasRevisionno ko 0 false*
dc.contributor.orcidDixon, Linda [0000-0003-3845-3016]es_ES
dc.contributor.orcidMontoya, María [0000-0002-5703-7360]es_ES
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