English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/210842
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:


A sensitive and rapid RNA silencing suppressor activity assay based on alfalfa mosaic virus expression vector

AuthorsMartínez-Pérez, Mireya; Navarro, José A. ; Pallás, Vicente; Sánchez-Navarro, Jesús A.
KeywordsRSS assay
P12 system
Issue Date15-Oct-2019
CitationVirus Research 272: 197733 (2019)
AbstractPlant viruses express RNA silencing suppressor (RSS) proteins to counteract plant defence mechanisms. Here, we describe a method to assess the RSS activity based on an alfalfa mosaic virus (AMV) RNA 3 expression vector and transgenic Nicotiana tabacum plants that express the P1 and P2 subunits of the AMV replicase (P12 plants). Inoculation of P12 plants with different AMV RNA 3 constructs expressing different HC-Pro mutants that differ in their RSS capabilities, revealed a perfect correlation between necrotic lesions on inoculated leaves and RSS activity. Protoplast analysis showed that the RSS activity correlated with the accumulation of the AMV RNAs. A direct comparison between three RSS activity assays and the AMV-P12 system revealed that the coat protein of carnation mottle virus displays RSS activity with the four assays and reduced the accumulation of the siRNAs.
Publisher version (URL)http://doi.org/10.1016/j.virusres.2019.197733
Identifiersdoi: 10.1016/j.virusres.2019.197733
issn: 0168-1702
Appears in Collections:(IBMCP) Artículos
(IBE) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.