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High-Resolution Atomic Force Microscopy Imagingof Nucleic Acids

AuthorsAres, Pablo; Gómez-Herrero, Julio; Moreno-Herrero, Fernando
KeywordsAtomic force microscopy
Double-stranded DNA
Double-stranded RNA
AFM imaging methods
Issue Date2018
PublisherHumana Press
CitationMethods in Molecular Biology 1814: 3-17 (2018)
AbstractExploring the limits of spatial resolution has been a constant in the history of atomic force microscopyimaging. Since its invention in 1986, the AFM has beaten the barrier of resolution continuously, thanks totechnical developments, miniaturization of tips, and implementation of new imaging modes. The doublehelix structure of DNA has been always at the horizon of resolution. Today, this milestone has beenreached, not only imaging DNA but also its close relative double-stranded RNA. Here, we provide acomprehensive description of the methods employed and the steps required to image the helical periodicityof these two nucleic acids with the sample immersed in a buffer solution.
Publisher version (URL)https://doi.org/10.1007/978-1-4939-8591-3_1
Appears in Collections:(CNB) Libros y partes de libros
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