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The group zero of gene regulatory evolution: Unveiling general principles of first-time cis-regulatory interactions between mammalian-specific TFS and the zebrafish genome

AuthorsLópez-Fernández, María José; Firbas, Panos; Maeso, Ignacio
Issue Date23-Oct-2019
CitationEuropean Developmental Biology Congress (2019)
AbstractBiological entities, whether proteins, blue whales, sensory organs or cis-regulatory elements are continuously interacting with other structures, establishing complex networks through which biological information is exchanged. These interaction networks, such as the complex gene regulatory networks that control animal development, are assembled and maintained during the course of evolution. But what happens when new elements are incorporated for the first time into these systems, when different biological structures meet for the first time? To answer this, we have artificially put in contact molecular structures that never overlapped before, to generate first time gene regulatory encounters. We have expressed highly divergent mammalian-specific homedomain transcription factors (TFs), such as ARGFX and LEUTX, in the completely foreign chromatin environment of zebrafish embryos. We perform ChIP-seq experiments for these mammalian TFs and show that their ectopic binding sites in zebrafish are preferentially found in chromatin regions that are open and accessible during zebrafish development, such as active promoters and enhancers. Surprisingly, despite the highly divergent recognition motifs of these mammalian TFs, some of their ectopic zebrafish binding sites are found in the zebrafish orthologs of ARGFX endogenous target genes in mammals. Our results suggest that, in evolution, first time molecular encounters can exhibit identifiable and coherent interaction patterns. Thus, biological molecular structures, such as TFs, have inherent capacities to interpret and read the information contained in other biological systems, even when these systems have not co-evolved with and are foreign to these biological structures
DescriptionResumen del trabajo presentado en el European Developmental Biology Congress, celebrado en Alicante (España), del 23 al 26 de octubre de 2019
Appears in Collections:(CABD) Comunicaciones congresos
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