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Title

Searching for additional functions on the Ribosome Biogenesis Nop7 complex

AuthorsOrea-Ordóñez, Lidia; Bravo, Jerónimo
Issue Date11-Dec-2019
Citation12ª Reunión de la Red Española de Levaduras (2019)
AbstractDuring eukaryotic ribosome biogenesis more than 200 factors remodel the incipient ribonucleoprotein without being part of the mature ribosome. Some of these factors are required for the synthesis of the ribosome like the Nop7 complex, essential for the synthesis of the 60S subunit. Recent structures of pre-ribome particles open some questions concerning the incorporation and release of the Nop7 complex to the preribosome. This heterotrimer is composed of Nop7, Ytm1 and Erb1 and exists as an independent complex before its incorporation to the immature particle. In vitro crosslinking experiments and biophysical evidences suggest that the trimer might have a different conformation as the one observed in the partial structures associated to pre-ribosomes. This possibility opens new questions about the functions exerted by the complex. In order to investigate these aspects we have performed tandem affinity purification coupled to mass spectrometry using Nop7 and Ytm1 tagged ultracentrifuged samples. Preliminary results are presented and an initial analysis of the proteins detected suggests insights into the Nop7 complex mechanism of action in the preribosome and additional functions non directly related with the ribosomal particles.
Description1 página. Abstract del trabajo presentado en 12ª Reunión de la Red Española de Levaduras. El Escorial, Madrid.11-13 de diciembre de 2019
URIhttp://hdl.handle.net/10261/210567
Appears in Collections:(IBV) Comunicaciones congresos
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