English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/210415
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


The ant Lasius niger is a new source of bacterial enzymes with biotechnological potential for bleaching dye

AuthorsDíez Méndez, Alexandra; García Fraile, Paula; Solano, Francisco; Rivas, Raúl
Azo dyes
Textile effluent
Issue Date2019
PublisherSpringer Nature
CitationScientific Reports 9: 15217 (2019)
AbstractIndustrial synthetic dyes cause health and environmental problems. This work describes the isolation of 84 bacterial strains from the midgut of the Lasius niger ant and the evaluation of their potential application in dye bioremediation. Strains were identified and classified as judged by rRNA 16S. The most abundant isolates were found to belong to Actinobacteria (49%) and Firmicutes (47.2%). We analyzed the content in laccase, azoreductase and peroxidase activities and their ability to degrade three known dyes (azo, thiazine and anthraquinone) with different chemical structures. Strain Ln26 (identified as Brevibacterium permense) strongly decolorized the three dyes tested at different conditions. Strain Ln78 (Streptomyces ambofaciens) exhibited a high level of activity in the presence of Toluidine Blue (TB). It was determined that 8.5 was the optimal pH for these two strains, the optimal temperature conditions ranged between 22 and 37 °C, and acidic pHs and temperatures around 50 °C caused enzyme inactivation. Finally, the genome of the most promising candidate (Ln26, approximately 4.2 Mb in size) was sequenced. Genes coding for two DyP-type peroxidases, one laccase and one azoreductase were identified and account for the ability of this strain to effectively oxidize a variety of dyes with different chemical structures.
Description11 páginas, 3 tablas, 2 figuras. -- The first publication is available at https://www.nature.com
Publisher version (URL)http://dx.doi.org/10.1038/s41598-019-51669-w
Appears in Collections:(IRNASA) Artículos
Files in This Item:
File Description SizeFormat 
the ant lasius niger.pdfArtículo principal1,24 MBAdobe PDFThumbnail
Show full item record
Review this work

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.