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Virulence Factors Expression In Staphylococcus Aureus Derived From Lower Respiratory Tract Infections And Their Role On Pathogenicity

AuthorsGodoy, Gerard; Lacoma, Alicia; Muriel, Beatriz; Montagut, Enric; Marco, María Pilar ; Prat, Cristina
Issue Date27-Sep-2019
AbstractObjectives 1. To evaluate Fibronectin-binding proteins (FnBPs) expression on clinical isolates of S. aureus derived from lower respiratory tract infections (LRTI). 2. To investigate the correlation between FnBPs expression and the ability to adhere and invade epithelial cells. 3. To develop an immunochemical test to detect quorum sensing (QS) molecules. Methods Purified fibronectin (Fn) was used to cover 96-well culture plates to allow bacteria attachment and was then quantified using crystal violet. Strains (n=63) were grown in tryptic soy broth till early exponential growth phase. To determine the capacity of cell adhesion and invasion, epithelial cells (A549) were incubated with different strains (n=15). A microtiter-based ELISA has been developed and used to detect autoinducible peptide (AIP) in bacterial supernatants. Results The strains with lower ability to adhere to Fn presented higher cell adhesion capacity in comparison to the ones that showed strong binding to Fn. Moreover, the latter strains had lower ability to be internalized by epithelial cells. AIP molecules were detected in supernatants, and its concentration increased over time. Conclusions 1. The expression profile of virulence factors can be associated with clinical phenotypes. 2. The presence of FnBPs increases the internalization capacity and its absence might increase the expression of adhesion proteins, thereby promoting cell adherence. 3. The detection of QS molecules could provide more information about the disease status and their role as biomarkers of disease should be evaluated.
Appears in Collections:(IQAC) Comunicaciones congresos
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