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Título: | Nanomaterials, screen printed carbon electrodes and their use for immunoassay development in the area of food safety |
Autor: | Kopper, Klaudia CSIC; Migliorelli, Davide; Galvé, Roger CSIC ORCID ; Marco, María Pilar CSIC ORCID CVN | Fecha de publicación: | 5-nov-2019 | Resumen: | Nowadays there is a high demand for cost-effective and user-friendly on-site screening tools in the food safety industry. Electrochemical sensors are the perfect candidates for this purpose since they combine the qualities of being miniaturized and relatively cheap at the same time. The use of carbon-based screen-printed electrodes (SPEs) is particularly ideal for on-site analysis, because of their well known low production cost, which drastically decreases the costs of the analysis. Furthermore, these SPEs can easily be modified with nanomaterials, which can result in a significant increase of their performance. In this study a characterization of in-house SPEs modified with CB and their application for the detection of different pesticides is described. Different materials have been investigated during the printing process and the working electrode has been modified with nanoparticles directly in the ink or by dropcasting either manually or using the Biodot dispenser. After an electrochemical characterization, the best performing SPE has been selected and its performance has been evaluated by using it either directly as a platform for the indirect competitive immunoassay or just as a transducer when the assay was performed using magnetic beads (MBs) as a platform for an Enzyme-Linked-Immunomagnetic-Electrochemical (ELIME) format. Both of the approaches have shown similar LOD and comparable sensitivity despite the fact that different enzymes (horseradish peroxidase and alkaline phosphatase) and different electrochemical techniques (Chronamperometry and Differential Pulse Voltammetry respectively) were applied, from which the latter approach also allows for multiplexation. Thus, the described system is a promising candidate for the development of a portable smartphone-connected device for on-site analysis. | URI: | http://hdl.handle.net/10261/210083 |
Aparece en las colecciones: | (IQAC) Comunicaciones congresos |
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