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http://hdl.handle.net/10261/209833
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Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Viguera, Enrique | es_ES |
dc.contributor.author | Rodríguez, A. | es_ES |
dc.contributor.author | Hernandez, Pablo | es_ES |
dc.contributor.author | Krimer, Dora B. | es_ES |
dc.contributor.author | Trellez. O. | es_ES |
dc.contributor.author | Schvartzman, Jorge Bernardo | es_ES |
dc.date.accessioned | 2020-04-30T13:07:57Z | - |
dc.date.available | 2020-04-30T13:07:57Z | - |
dc.date.issued | 1998-09-14 | - |
dc.identifier.citation | Gene 217(1-2) 41-49 (1998) | es_ES |
dc.identifier.issn | 0378-1119 | - |
dc.identifier.uri | http://hdl.handle.net/10261/209833 | - |
dc.description | 9 p.-6 fig. | es_ES |
dc.description.abstract | We present a computer model to predict the patterns expected for the replication intermediates (RIs) of DNA fragments analyzed by neutral/neutral two-dimensional (2D) agarose gel electrophoresis. The model relies on the mode of replication (uni- or bi-directional), the electrophoretic mobility of linear DNA fragments and the retardation caused by the three-dimensional shape of non-linear molecules. The utility of this model is demonstrated with two examples: replication analysis of the plasmids pBR322 and pHH5.8 in Escherichia coli after digestions with EcoRI and HindIII, respectively. | es_ES |
dc.description.sponsorship | This work was partially supported by Grant 96/0470 from the Spanish Fondo de Investigación Sanitaria,Grant PM95/0016 from the Spanish Dirección General de Enseñanza Superior,Grant 08.6/0016/1997 from the Comunidad Autónoma de Madrid and Grant TIC96-1125-C03-01 from the Spanish Comisión Interministerial de Ciencia y Tecnología (CICYT ). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.rights | closedAccess | es_ES |
dc.subject | 2D agarose gel electrophoresis | es_ES |
dc.subject | DNA replication | es_ES |
dc.subject | Replication intermediates | es_ES |
dc.subject | Origins | es_ES |
dc.subject | Termini | es_ES |
dc.subject | Replication fork barriers (RFBs) | es_ES |
dc.title | A computer model for the analysis of DNA replication intermediates by two-dimensional agarose gel electrophoresis | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1016/s0378-1119(98)00375-8 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | https://doi.org/10.1016/s0378-1119(98)00375-8 | es_ES |
dc.identifier.e-issn | 1879-0038 | - |
dc.contributor.funder | Instituto de Salud Carlos III | es_ES |
dc.contributor.funder | Dirección General de Enseñanza Superior e Investigación Científica (España) | es_ES |
dc.contributor.funder | Comunidad de Madrid | es_ES |
dc.contributor.funder | Comisión Interministerial de Ciencia y Tecnología, CICYT (España) | es_ES |
dc.relation.csic | Sí | es_ES |
oprm.item.hasRevision | no ko 0 false | * |
dc.identifier.funder | http://dx.doi.org/10.13039/100012818 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100004587 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100007273 | es_ES |
dc.contributor.orcid | Viguera, Enrique [0000-0001-5475-3807] | es_ES |
dc.contributor.orcid | Hernandez, Pablo [0000-0002-8113-1277] | es_ES |
dc.contributor.orcid | Krimer, Dora B. [0000-0001-9972-3994] | es_ES |
dc.contributor.orcid | Schvartzman, Jorge Bernardo [0000-0002-1856-6278] | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | No Fulltext | - |
item.languageiso639-1 | en | - |
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