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Title

A computer model for the analysis of DNA replication intermediates by two-dimensional agarose gel electrophoresis

AuthorsViguera, Enrique; Rodríguez, A.; Hernandez, Pablo ; Krimer, Dora B. ; Trellez. O.; Schvartzman, Jorge Bernardo
Keywords2D agarose gel electrophoresis
DNA replication
Replication intermediates
Origins
Termini
Replication fork barriers (RFBs)
Issue Date14-Sep-1998
PublisherElsevier
CitationGene 217(1-2) 41-49 (1998)
AbstractWe present a computer model to predict the patterns expected for the replication intermediates (RIs) of DNA fragments analyzed by neutral/neutral two-dimensional (2D) agarose gel electrophoresis. The model relies on the mode of replication (uni- or bi-directional), the electrophoretic mobility of linear DNA fragments and the retardation caused by the three-dimensional shape of non-linear molecules. The utility of this model is demonstrated with two examples: replication analysis of the plasmids pBR322 and pHH5.8 in Escherichia coli after digestions with EcoRI and HindIII, respectively.
Description9 p.-6 fig.
Publisher version (URL)https://doi.org/10.1016/s0378-1119(98)00375-8
URIhttp://hdl.handle.net/10261/209833
DOI10.1016/s0378-1119(98)00375-8
ISSN0378-1119
E-ISSN1879-0038
Appears in Collections:(CIB) Artículos
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