English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/209833
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:


A computer model for the analysis of DNA replication intermediates by two-dimensional agarose gel electrophoresis

AuthorsViguera, Enrique; Rodríguez, A.; Hernandez, Pablo ; Krimer, Dora B. ; Trellez. O.; Schvartzman, Jorge Bernardo
Keywords2D agarose gel electrophoresis
DNA replication
Replication intermediates
Replication fork barriers (RFBs)
Issue Date14-Sep-1998
CitationGene 217(1-2) 41-49 (1998)
AbstractWe present a computer model to predict the patterns expected for the replication intermediates (RIs) of DNA fragments analyzed by neutral/neutral two-dimensional (2D) agarose gel electrophoresis. The model relies on the mode of replication (uni- or bi-directional), the electrophoretic mobility of linear DNA fragments and the retardation caused by the three-dimensional shape of non-linear molecules. The utility of this model is demonstrated with two examples: replication analysis of the plasmids pBR322 and pHH5.8 in Escherichia coli after digestions with EcoRI and HindIII, respectively.
Description9 p.-6 fig.
Publisher version (URL)https://doi.org/10.1016/s0378-1119(98)00375-8
Appears in Collections:(CIB) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdfRestringido15,38 kBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.