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Title

An “omic” approach to Pyrocystis lunula: New insights related with this bioluminescent dinoflagellate

AuthorsFajardo, Carlos; Amil-Ruiz, Francisco; Fuentes-Almagro, Carlos; Donato, Marcos; Martínez-Rodríguez, Gonzalo ; Escobar-Niño, Almudena; Carrasco, Rafael; Mancera, Juan Miguel; Fernandez-Acero, Francisco Javier
KeywordsP. lunula
Transcriptomics
Proteomics
Luciferin-binding protein
Glutathione-S-transferase
Bioluminescence
Issue Date30-Oct-2019
PublisherElsevier
CitationJournal of Proteomics 209: 103502 (2019)
AbstractPyrocystis lunula (Schutt) is a photoautotrophic dinoflagellate without armored form, frequently found in marine environments. Today, there are several biotechnological applications derived from the bioluminescent system of this species. From a post-genomic perspective, in order to have a starting point for studying the proteome of P. lunula, an “omics” approach (transcriptomics-proteomics) was assessed using fresh microalgae samples. A total of 80,874,825 raw reads were generated (11,292,087,505 bp; 55.82% GC) by mRNA sequencing. Very high-quality sequences were assembled into 414,295 contigs (219,203,407 bp; 55.38% GC) using Trinity software, generating a comprehensive reference transcriptome for this species. Then, a P. lunula proteome was inferred and further employed for its analysis on this species. A total of 17,461 peptides were identified, yielding 3182 protein identification hits, including 175 novel proteins. The identified proteins were further categorized according to functional description and gene ontology classification. Significance: The major contribution of the present work is making available a reference transcriptome and proteome of P. lunula, that is now accessible for the research community, and a functional description of the 3182 proteins inferred from the transcriptome, including 175 novel proteins, which have already been deposited in the ProteomeXchange and NCBI SRA databases, respectively. In addition to this, a series of important factors related to the bioluminescent system and the regulation of gene expression, were identified and described.
Publisher version (URL)http://doi.org/10.1016/j.jprot.2019.103502
URIhttp://hdl.handle.net/10261/209695
DOI10.1016/j.jprot.2019.103502
Identifiersdoi: 10.1016/j.jprot.2019.103502
e-issn: 1876-7737
issn: 1874-3919
Appears in Collections:(ICMAN) Artículos
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