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Subcellular localization and nitric oxide-scavenging activity of plant hemoglobins

AuthorsRubio Luna, María Carmen ; Calvo-Begueria, Laura; Díaz-Mendoza, M. ; Elhi, M.; Moore, M.; Matamoros Galindo, Manuel Ángel ; James, E. K.; Díaz, I.; Pérez-Rontomé, Carmen ; Villar Rua, Irene ; Sein-Echaluce, Violeta C.; Hebelstrup, K. H.; Dietz, K. J.; Becana Ausejo, Manuel
Issue DateJul-2019
Citation14th International Conference on Reactive Oxygen and Nitrogen Species in Plants (Munich, Germany. July 10-12, 2019)
AbstractSymbio􀆟c hemoglobins provide O2 to N2-fixing bacteria within legume nodules. However, the func􀆟ons of nonsymbio􀆟c hemoglobins or phytoglobins (Glbs) are less defined. Three Glb classes can be dis􀆟nguished based on phylogene􀆟c and biochemical analyses and may coexist in plant 􀆟ssues: class 1 Glbs have extreme O2 affinity and are induced by hypoxia; class 2 Glbs have moderate O2 affinity and are precursors of leghemoglobins; and class 3 have low O2 affinity and high sequence homology with bacterial truncated hemoglobins. Immunogold labeling combined with confocal microscopy of Glbs tagged with GFP at the C-terminus was used to determine the subcellular localiza􀆟ons of Glbs in the model plants Arabidopsis and Lotus japonicus. To this end, we used overexpressing and knockout or silenced lines of Arabidopsis, performed quan􀆟ta􀆟ve immunolabeling, and monitored the GFP-tagged proteins in leaf cells and protoplasts.
Description1 Pag.
Appears in Collections:(EEAD) Comunicaciones congresos
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