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Effects of Halophyte Root Exudates and Their Components on Chemotaxis, Biofilm Formation and Colonization of the Halophilic Bacterium Halomonas Anticariensis FP35T

AuthorsSampedro, Inmaculada; Pérez-Mendoza, Daniel; Toral, Laura; Palacios, Esther; Arriagada, César; Llamas, Inmaculada
Issue Date16-Apr-2020
PublisherMultidisciplinary Digital Publishing Institute
CitationMicroorganisms 8 (4): 575 (2020)
AbstractIncrease in soil salinity poses an enormous problem for agriculture and highlights the need for sustainable crop production solutions. Plant growth-promoting bacteria can be used to boost the growth of halophytes in saline soils. <i>Salicornia</i> is considered to be a promising salt-accumulating halophyte for capturing large amounts of carbon from the atmosphere. In addition, colonization and chemotaxis could play an important role in <i>Salicornia</i>-microbe interactions. In this study, the role of chemotaxis in the colonization of the halophilic siredophore-producing bacteria, <i>Halomonas anticariensis</i> FP35<sup>T</sup>, on <i>Salicornia hispanica</i> plants was investigated. The chemotactic response of FP35<sup>T</sup> to <i>Salicornia</i> root exudates showed optimum dependence at a salt concentration of 5 % NaCl (w/v). Oleanolic acid, the predominant compound in the exudates detected by HPLC and identified by UPLC-HRMS Q-TOF, acts as a chemoattractant. In vitro experiments demonstrated the enhanced positive effects of wild-type <i>H. anticariensis</i> strain FP35<sup>T</sup> on root length, shoot length, germination and the vigour index of <i>S. hispanica.</i> Furthermore, these positive effects partially depend on an active chemotaxis system, as the chemotaxis mutant <i>H. anticariensis</i> FP35 &Delta;cheA showed reduced plant growth promotion for all the parameters tested. Overall, our results suggest that chemotaxis responses to root exudates play an important role in interactions between <i>Salicornia</i> and halophilic bacteria, enhance their colonization and boost plant growth promotion. Preliminary results also indicate that root exudates have a positive impact on <i>H. anticariensis</i> FP35<sup>T</sup> biofilm formation under saline conditions, an effect which totally depends on the presence of the <i>cheA</i> gene.
Identifiersdoi: 10.3390/microorganisms8040575
Appears in Collections:Colección MDPI
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