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dc.contributor.authorPech-Canul, Ángel de la Cruz-
dc.contributor.authorRivera-Hernández, Geovanny-
dc.contributor.authorNogales, Joaquina-
dc.contributor.authorGeiger, Otto-
dc.contributor.authorSoto, María José-
dc.contributor.authorLópez-Lara, Isabel M.-
dc.date.accessioned2020-04-28T14:07:10Z-
dc.date.available2020-04-28T14:07:10Z-
dc.date.issued2020-03-26-
dc.identifier.citationMicroorganisms 8(4): 470 (2020)-
dc.identifier.issn2076-2607-
dc.identifier.urihttp://hdl.handle.net/10261/209464-
dc.description© 2020 by the authors.-
dc.description.abstractFadD is an acyl-coenzyme A (CoA) synthetase specific for long-chain fatty acids (LCFA). Strains mutated in fadD cannot produce acyl-CoA and thus cannot grow on exogenous LCFA as the sole carbon source. Mutants in the fadD (smc02162) of Sinorhizobium meliloti are unable to grow on oleate as the sole carbon source and present an increased surface motility and accumulation of free fatty acids at the entry of the stationary phase of growth. In this study, we found that constitutive expression of the closest FadD homologues of S. meliloti, encoded by sma0150 and smb20650, could not revert any of the mutant phenotypes. In contrast, the expression of Escherichia coli fadD could restore the same functions as S. meliloti fadD. Previously, we demonstrated that FadD is required for the degradation of endogenous fatty acids released from membrane lipids. Here, we show that absence of a functional fadD provokes a significant loss of viability in cultures of E. coli and of S. meliloti in the stationary phase, demonstrating a crucial role of fatty acid degradation in survival capacity.-
dc.description.sponsorshipThis research was funded by grants from CONACyT/Mexico (153998 and 253549), from the Spanish Ministry /MINECO/MCIU/AEI (BIO2013-42801-P and PGC2018-096477-B-I00) and FEDER funds (EU). The APC was funded by Universidad Nacional Autónoma de México. G.R-H was supported by a postdoctoral fellowship from the DGAPA (Dirección General de Asuntos del Personal Académico)-UNAM (Universidad Nacional Autónoma de México). Á.d.l.C.P.-C. was supported by CONACyT through the “Cátedras CONACyT para Jóvenes Investigadores” Programme (Project #609).-
dc.publisherMultidisciplinary Digital Publishing Institute-
dc.relationMINECO/ICTI2013-2016/BIO2013-42801-P-
dc.relationMICIU/ICTI2017-2020/PGC2018-096477-B-I00-
dc.relationPGC2018-096477-B-I00/AEI/10.13039/501100011033-
dc.relation.isversionofPublisher’s version-
dc.rightsopenAccess-
dc.subjectLong-chain acyl-CoA synthetase-
dc.subjectFree fatty acids-
dc.subjectSinorhizobium (Ensifer)-
dc.subjectSurface motility-
dc.subjectSurvival-
dc.subjectMalonyl-CoA synthetase-
dc.titleRole of Sinorhizobium meliloti and Escherichia coli Long-Chain Acyl-CoA Synthetase FadD in Long-Term Survival-
dc.typeartículo-
dc.identifier.doi10.3390/microorganisms8040470-
dc.description.peerreviewedPeer reviewed-
dc.relation.publisherversionhttps://doi.org/10.3390/microorganisms8040470-
dc.identifier.e-issn2076-2607-
dc.date.updated2020-04-28T14:07:10Z-
dc.rights.licensehttp://creativecommons.org/licenses/by/4.0/-
dc.contributor.funderMinisterio de Economía y Competitividad (España)-
dc.contributor.funderMinisterio de Ciencia, Innovación y Universidades (España)-
dc.contributor.funderAgencia Estatal de Investigación (España)-
dc.contributor.funderConsejo Nacional de Ciencia y Tecnología (México)-
dc.contributor.funderEuropean Commission-
dc.contributor.funderUniversidad Nacional Autónoma de México-
dc.relation.csic-
dc.identifier.funderhttp://dx.doi.org/10.13039/501100011033es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003141es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000780es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100005739es_ES
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