English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/209383
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


Effects of the microtubule nucleator Mto1 on chromosomal movement, DNA repair, and sister chromatid cohesion in fission yeast.

AuthorsZhurinsky, Jacob; Salas-Pino, Silvia ; Iglesias-Romero, Ana B.; Torres-Méndez, Antonio; Knapp, Benjamin; Flor-Parra, Ignacio; Wang, Jiyong; Bao, Kehan; Songtao, Jia; Chang, Fred; Daga, Rafael R.
Issue Date1-Oct-2019
PublisherAmerican Society for Cell Biology
CitationMolecular Biology of the Cell 30(21): 2695-2708 (2019)
AbstractAlthough the function of microtubules (MTs) in chromosomal segregation during mitosis is well characterized, much less is known about the role of MTs in chromosomal functions during interphase. In the fission yeast Schizosaccharomyces pombe, dynamic cytoplasmic MT bundles move chromosomes in an oscillatory manner during interphase via linkages through the nuclear envelope (NE) at the spindle pole body (SPB) and other sites. Mto1 is a cytoplasmic factor that mediates the nucleation and attachment of cytoplasmic MTs to the nucleus. Here, we test the function of these cytoplasmic MTs and Mto1 on DNA repair and recombination during interphase. We find that mto1Δ cells exhibit defects in DNA repair and homologous recombination (HR) and abnormal DNA repair factory dynamics. In these cells, sister chromatids are not properly paired, and binding of Rad21 cohesin subunit along chromosomal arms is reduced. Our findings suggest a model in which cytoplasmic MTs and Mto1 facilitate efficient DNA repair and HR by promoting dynamic chromosomal organization and cohesion in the nucleus.
Publisher version (URL)http://dx.doi.org/10.1091/mbc.E19-05-0301
Appears in Collections:(CABD) Artículos
Files in This Item:
File Description SizeFormat 
Effects _Zhurinskya_Art2019.pdf2,5 MBUnknownView/Open
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.