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Nitric oxide production in legume nodules examined by combining electron paramagnetic resonance and fluorescent probes

AuthorsRubio Luna, María Carmen ; Calvo-Begueria, Laura; Martínez, J. I.; Pérez-Rontomé, Carmen ; Becana Ausejo, Manuel
Issue DateJul-2019
PublisherSociedad Española de Fijación de Nitrógeno
CitationXVII Reunión Nacional de la Sociedad Española de Fijación de Nitrógeno (Madrid. 10 julio 2019)
VI Portuguese - Spanish Congress on Nitrogen Fixation (Madrid. July 10, 2019)
AbstractNitric oxide (NO) is a gaseous free radical and signaling molecule with multiple functions in plants, including the onset of the legume-rhizobia symbiosis and the development and senescence of root nodules (Hichri et al., 2016). Some of these functions may rest on NO acting as intermediate in the signaling cascades of most phytohormones (Hill, 2012). To gain insight into the functions of NO in nodules, it is therefore crucial to localize the sites of NO production and accumulation at the tissue, cell, and organelle levels. For this purpose, we have employed two different techniques: electron paramagnetic resonance (EPR) spectroscopy and the specific fluorescent dye 4,5-diaminofluorescein diacetate (DAF-2 DA). The EPR detects the highly stable nitrosyl-leghemoglobin (Lb2+NO) complex, while the DAF-2 DA probe detects free NO. Also, we have used Bradyrhizobium diazoefficiens mutant strains lacking the enzymes of the denitrification pathway: nitrate reductase (Nap), nitrite reductase (NirK), or nitric oxide reductase (Nor) (Meakin et al., 2007).
Description1 Pag.
Appears in Collections:(EEAD) Comunicaciones congresos
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