English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/209009
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Contrasting outcomes of Vibrio harveyi pathogenicity in gilthead seabream, Sparus aurata and European seabass, Dicentrachus labrax

AuthorsFirmino, Joana; Furones, M. Dolores; Andree, Karl B.; Sarasquete, Carmen ; Ortiz-Delgado, Juan B. ; Asencio-Alcudia, Gloria; Gisbert, Enric
KeywordsVibrio harveyi
Sparus aurata
Dicentrachus labrax
Abdominal swelling
Bacterial challenge
Co-habitation trial
Ascites
Issue Date15-Sep-2019
PublisherElsevier
CitationAquaculture 511: 734210 (2019)
AbstractVibrio harveyi has been reported as the dominant heterotrophic bacterial species in western Mediterranean coastal areas during warm seasons, and is recognized as an economically significant pathogen for the aquaculture industry. The present work aimed to evaluate the pathogenicity of a V. harveyi strain isolated from ascitic fluid collected from cultured gilthead seabream and then used in a challenge experiment involving the two most important fish species in Mediterranean aquaculture: gilthead seabream, Sparus aurata and European seabass, Dicentrarchus labrax. The ascitic fluid from diseased juvenile seabreams, previously vaccinated against Photobacterium damselae and Vibrio anguillarum, was extracted and bacteria cultivated for isolation and characterization. Additionally, different tissues were sampled for histological evaluation and description. Significant histopathological responses were observed in hepatic and mucosal tissues. One of the strains isolated from ascitic fluid, IRTA 17-43, was selected for a bacterial challenge. Additionally, the attenuation of virulence through sequential passage of the strain on solid media was also assessed. In parallel, a co-habitation trial was performed in order to evaluate the possible transfer of the bacteria between injected and healthy individuals. Pathogenicity trials in gilthead seabream resulted in only 25% mortality when injected with 10 CFU mL, whereas, for European seabass, a mortality of 95% was recorded, with clear signs of vibriosis. When passed sequentially on solid media, the strain IRTA-17-43 showed a decrease of 35% in cumulative mortality for European seabass. No apparent transmission of the pathogen occurred during the co-habitation trial for both species. In conclusion, although few external signs of V. harveyi are observed in vaccinated carriers, internal effects of the infection were clear and severe. Although no horizontal transfer of infection was observed, the risk of occurrence between carriers and immunosuppressed individuals or between different species should be considered. This further validates that the establishment of a good health management system within fish farms is of major importance in order to avoid the onset of disease outbreaks.
Publisher version (URL)http://doi.org/10.1016/j.aquaculture.2019.734210
URIhttp://hdl.handle.net/10261/209009
Identifiersdoi: 10.1016/j.aquaculture.2019.734210
issn: 0044-8486
Appears in Collections:(ICMAN) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.