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dc.contributor.authorSánchez González, Ramón-
dc.contributor.authorBribian, A.-
dc.contributor.authorSalvador Cabos, Nieves-
dc.contributor.authorLópez-Mascaraque, Laura-
dc.date.accessioned2020-04-23T16:53:30Z-
dc.date.available2020-04-23T16:53:30Z-
dc.date.issued2019-07-10-
dc.identifier.citationGLIA 67:E125¿E766 (2019) : T02-048B-
dc.identifier.urihttp://hdl.handle.net/10261/208886-
dc.descriptionGLIA 67:E125¿E766 (2019) : T02-048B-
dc.description.abstractNG2-cells are the most enigmatic neural population, homogenously distributed in a grid-like manner in both gray and white matter regions of the adult brain. In the CNS, a fraction of NG2 cells (OPCs) is responsible for the generation of mature oligodendrocytes, but most of them do not differentiate, representing the most proliferative cells out of the adult neurogenic niches. Fate-mapping analysis in different transgenic mice lines revealed different degrees of differentiation and maturation properties of NG2 cells depending on brain areas. Such diversity suggests that NG2-cells conforms a heterogeneous population composed by different subpopulations devoted to distinct functions. Our hypothesis is that those differences emerge during development as occurs with other glial cell subtypes that are generated from specific progenitors (García-Marques and Lopez-Mascaraque, 2013; 2017). Using a multi-color genetic lineage tracing system, StarTrack, we specifically targeted pallial progenitors, to analyze the type and location of adult labelled cells related to their origin and embryonic stage. Star Track labelled cells exhibited different neural phenotypes and located at different regions that were classified in relation to the targeted embryonic area. Moreover, we also designed different StarTrack strategies to permanently label the individual progenitor cells and their progeny. The plasmids are injected in the lateral ventricles and electroporared in vivo using in utero (IUE) or postnatal electroporation at different stages (E12-P1). First, NG2-EGFP-StarTrack was used to specifically label their cell progeny. Preliminary data showed that labeled cells are located in the gray and white matter, and those cells represent different neural types. Secondly, to specific target the NG2-progenitors we performed the same experiment using Ubc-StarTrack along with an embodying NG2 promoter into the transposase. Preliminary results showed that those progenitors cells labeled with UbC-EGFP-StarTrack constructs are committed to give rise to astrocytes, NG2 cells and even neurons at P90. We expect that our findings will provide fundamental aspects of the lineage potential, cell fate determination and functionality of NG2 cells.-
dc.description.sponsorshipSupported by research Grant BFU2016-75207-R from MINECO-
dc.languageeng-
dc.publisherJohn Wiley & Sons-
dc.relationMINECO/ICTI2013-2016/BFU2016-75207-R-
dc.relation.isversionofPublisher's version-
dc.rightsopenAccess-
dc.titleProgenitor potential and cell progeny of NG2-glia-
dc.typepóster de congreso-
dc.date.updated2020-04-23T16:53:30Z-
dc.contributor.funderMinisterio de Economía y Competitividad (España)-
dc.relation.csic-
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
Appears in Collections:(IC) Comunicaciones congresos
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