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Title

Phenotyping of early stages of wild Helianthus species/ Orobanche cumana interaction towards the identification of new resistances

AuthorsChabaud, Mireille; Boniface, Marie-Claude; Folletti, Tifaine; Pérez-Vich, Begoña ; Velasco Varo, Leonardo ; Simier, Philippe; Delavault, Philippe; Muños, Stéphane
Issue Date2019
Citation15th World Congress on Parasitic Plants (2019)
AbstractOrobanche cumana(the sunflower broomrape) is an obligate parasitic plant that specifically infects sunflower and is one of the main constraint of sunflower crop in Europe. Genetic resistance is the most efficient control of this parasitic weed illustrated by the breeding for resistant varieties in the past decades. However, more virulent broomrape populations are frequently emerging in many countries by overcoming the resistances introgressed in the cultivated sunflower varieties. Building durable resistance requires the combination of various resistance mechanisms and origins, named pyramidal resistance (Velasco et al., 2016), in contrast to vertical monogenic resistance. Searching for new and complementary broomrape resistance sources and mechanisms is hence a priority for sunflower breeding. Wild Helianthus species have been shown to provide such resources (Seiler and Jan, 2014). With this purpose, a screen of wild Helianthus species has been undertaken, through phenotyping of various stages of their interaction with the most virulent races of O. cumana: broomrape seed germination and haustorium formation using Helianthus root exudates, early stages (attachment and tubercle development) in mini-rhizotrons, and emerged shoots in pots. For early stages, a phenotyping tool in mini-rhizotrons (Louarn et al., 2016) has been optimised through the set-up of the nutritive solution, image acquisition using a nano-computer Raspberry/ cameraPi, and image analysis using Image J software. Sixty accessions (wild Helianthus andwild H. annuus) including H. grosseserratus, H. tuberosus, H. anomalus, H. divaricatus, H. bolanderi, H. argophyllus, H. petiolaris, H. praecox, H. debilis, H. nuttallii, H. strumosus and H. pauciflorusare being screened in the project. First results will be presented including cytological studies.
Description15th World Congress on Parasitic Plants, 30th June–5th July, Amsterdam, the Netherland.
URIhttp://hdl.handle.net/10261/208842
Appears in Collections:(IAS) Comunicaciones congresos
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