English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/20883
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Título

Histophagous scuticociliatids (Ciliophora) parasitizing turbot (Scophthalmus maximus L.). Morphology, in vitro culture and virulence

AutorÁlvarez-Pellitero, Mª del Pilar ; Palenzuela, Oswaldo ; Padrós, Francesc; Sitjà-Bobadilla, Ariadna ; Riaza, Ana; Silva, Raquel; Arán, Javier
Palabras claveScuticociliatia
Ciliophora
turbot
in vitro culture
virulence
aquaculture
Psetta maxima
Fecha de publicación2004
CitaciónFolia Parasitologica 51:177-187
ResumenSystemic ciliatosis caused by histophagous ciliates constitutes a serious disease of cultured turbot. Six ciliate isolates were obtained from parasitized turbot during six epizootics at four different farms located in Spain, France and Portugal. Axenic cultures of the six isolates were obtained by periodical subculturing in ATCC 1651MA or supplemented L-15 media. In basal media or seawater, the parasites could survive starving for long periods with no apparent proliferation. In adequate media, growth kinetics was found to be very similar for isolates A and B, with a clear influence of temperature. Morphological studies demonstrated that all isolates share common features that allows their assignment to either Philasterides Kahl, 1931 or Miamiensis Thompson et Moewus, 1964. However, statistically significant differences were evident in pairwise comparisons of the isolates from the four farm sites in 16 taxonomically relevant morphometric features. This could allow the discrimination of different species or strains. Virulence of isolates A and B for healthy turbot was tested in several experiments. Differences in the virulence were especially evident after long-term in vitro culturing, isolate A being clearly attenuated after 35-42 passages, whereas isolate B became more virulent after 20-42 passages. The need of further studies to confirm such virulence variability and its implications in pathogenesis and prevention of turbot scuticociliatoses is stressed.
Versión del editorhttp://www.paru.cas.cz/folia/pdfs/showpdf.php?pdf=20708
URIhttp://hdl.handle.net/10261/20883
ISSN0015-5683
Aparece en las colecciones: (IATS) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Folia Par 2004 51 177-187.pdf1,25 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.