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One week high-fat diet alters MTR, T2 and ADC values in the mouse hypothalamus and reward centers

AuthorsCampillo, B. W.; Cerdán, Sebastián ; López-Larrubia, Pilar ; Lizarbe, Blanca
Issue Date3-Oct-2019
Citation37th Annual Meeting of European Society for Magnetic Resonance In Medicine and Biology (ESMRMB) (2019)
Abstract[Purpose/Introduction]: Obesity development is linked to inflammation in the hypothalamus, the brain structure responsible for appetite regulation and energy homeostasis. Furthermore, appetite has in mammals a hedonic component, controlled by the reward centers, affected during inflammation1. In rodents, inflammation has been characterized, at a histological level, by the emergence of microgliosis and astrogliosis, events that can be detected before gain in body weight. Studies using Magnetic Resonance Imaging (MRI) have identified a rise in the transverse relaxation time (T2) –a surrogate marker of astrocytosis- in the hypothalamus of long-term highfat diet (HFD) mice and in obese patients. Nevertheless, no MRI hypothalamic inflammatory markers of the initial stages of obesity have yet been investigated, and the role of the reward centers remains to be determined. Therefore, the aim of this study was to assess the onset of obesity development in mice in vivo using MRI. We evaluated the longitudinal changes of T2, magnetization transfer ratios (MTR) and apparent diffusion coefficients (ADC) in mice during the initial stages of high-fat or standard diet consumption.
[Subjects and Methods]: Two animal groups, fed with either standard food chow (n = 7) or with a 60% high-fat (butter-based) diet (n = 8) were investigated before diet diversification (T = 0) and on days 1, 7 and 14. Body weight and blood glucose levels were controlled at every time point. MRI were performed in a 7T magnet (Bruker Biospect). Briefly, the hypothalamus (Hyp), nucleus accumbens (ACB) and infralimbic area (ILA) were localized in a T2-image using an anatomical atlas, and T2 (50 images, TE: 12–600 ms,TR = 5000 ms), MTR (MT pulse on/off, TE/TR = 9.8/2500 ms) maps and DWI (9 b:200-2000 smm-2) were acquired (5 slices, 1.25 mm thickness, 0.16 9 0.16 mm2). Longitudinal changes of the fitted parameters were assessed statistically using SPSS and a generalized estimated equations model
[Results]: Body weight and blood glucose levels increased significantly on HFD mice from days 1 and 7, respectively, and control animals increased only body weight after 14 days (Figure 1).
DescriptionTrabajo presentado en el 37th Annual Meeting; European Society for Magnetic Resonance In Medicine and Biology (ESMRMB), celebrado en Rotterdam (Países Bajos) del 3 al 5 de octubre de 2019.
Appears in Collections:(IIBM) Comunicaciones congresos
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