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Magnetic separation and high reusability of chloroperoxidase entrapped in multi polysaccharide micro-supports

AuthorsGarcía-Embid, Sonia; Renzo, Francesca di; Matteis, Laura de; Spreti, Nicoletta; Fuente, Jesús M. de la
Issue Date2019
CitationE-MRS (2019)
AbstractThe World Commission on Environment and Development introduced the term sustainable development, indicating the present need of modern industrial processes to optimize the use of raw materials, reduce waste and avoid the use of toxic molecules. Amongst the approaches used over the years to achieve this sustainability, biocatalysts, especially enzymes, have been in the spotlight due to their great properties. Sustainability of enzymatic catalysis is maintained through the whole cycle: from their production (living organisms) to the waste treatment. However, their present application at industrial scale is hampered by the high costs in their production that decrease cost-effectiveness of their application. Reutilization of the enzyme is therefore the tool to obtain more cost-effective and sustainable industrial processes. Immobilization of these biocatalysts allows an easy recovery of the material and protection from the reaction conditions in the different production steps. Nowadays, nanotechnology offers one of the most forefront approaches for enzyme immobilization. Magnetic nanoparticles allow an easy recovery of an immobilized enzyme using a simple magnet to separate the catalyst from the reaction product. To improve colloidal stability of the support, reduce interactions between the magnetic cores and prevent interactions with the environment that can affect both support and enzyme stability, a polymer coating is an easy and cheap approach. Using this approach, in this work we developed a hybrid, modular micro-support based on organic and inorganic nanocomponents. The easiness of tuning the composition of the support makes this system a potentially universal support for the immobilization of very different catalytic systems. Here we present the application of the developed micro-support for the immobilization of chloroperoxidase (CPO), an enzyme able to catalyze many reactions of large-scale interest. A multipolysaccharidic shell containing the immobilized enzyme and obtained through a combination of chitosan and alginate, biodegradable polymers from natural sources, was used to stabilize a nanoemulsion core in which magnetic nanoparticles were embedded. Microsupports obtained through different combinations of nanocomponents were characterized and tested in terms of their chemical stability under reaction conditions. An excellent reusability of the entrapped enzyme was observed opening the way to the immobilization of different catalytic systems and to the scale-up study in view of future industrial application.
DescriptionResumen del trabajo presentado al 37th Spring Meeting of the European Materials Research Society (E-MRS), celebrado del 27 al 31 de mayo de 2019 en Niza (Francia).
Appears in Collections:(ICMA) Comunicaciones congresos
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