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Gold nanoprisms as transducers for the development of plasmonic photothermal biosensors

Other TitlesPlasmonic nanoparticles for thermal detection of cancer biomarkers
AuthorsMartín-Barreiro, Alba; Moros, María; Grazú, Valeria; Fuente, Jesús M. de la
Issue Date2019
Citation2nd Spanish Conference on Biomedical Applications of Nanomaterials (2019)
AbstractGold nanoprisms (AuNPrs) are anisotropic nanoparticles that exhibit localized surface plasmon resonance (LSRP) in the near infrared (NIR) region. This optical property allows low concentrations of AuNPrs to convert light energy into quantifiable heat (photothermal effect) and makes their application interesting in the development of biological assays. To evaluate the characteristics of these materials for this purpose and to study possible transduction-detection methods are the objective of this work. For this, different types of NPrs have been synthesized and characterized. The recording of the heat released has been detected with an IR camera and this thermal signals have been evaluated by different treatment methods (temperature variation, photothermal conversion efficiency and emission-decay signal area). In addition, the use of thermosensitive paper has been coupled to this methodology, in order to amplify the thermal signal and translate it into a colorimetric signal (gray scale) easily quantifiable. In this way, AuNPrs linked to a biological receptor can act as physical-chemical transducers, interpreting the biological recognition with the analyte as a quantifiable thermal signal, that can be detected with an IR camera. Thus, a ThermoELISA sandwich immunoassay for the thermal detection of the tumor marker carcinoembryonic antigen (CEA) was developed and optimized. For this goal, the union of the detection antibody (Ab_det) with AuNPrs have been optimized by means of the oriented binding AuNPrs@streptavidin – biotin@Ab_det. This strategy provides a versatile bioconjugate that can be subsequently linked to any biotinylated antibody which allows extending the methodology to other cancer biomarkers. Final ThermoELISA developed greatly increases the sensitivity with respect to the classic colorimetric ELISA and decreases the limits of detection (0.91 ng/mL) and quantification (3.03 ng/mL) with a low RSD (13%) and a less time-consuming.
DescriptionResumen del póster presentado a la 2nd Spanish Conference on Biomedical Applications of Nanomaterials (SBAN), celebrada en Madrid del 6 al 7 de junio de 2019.
Appears in Collections:(ICMA) Comunicaciones congresos
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