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Study of the interaction between polysaccharide nanocapsules and nasal mucosa epithelium for the intranasal administration of drugs for Alzheimer's disease

AuthorsAlleva, María; Moros, María; Matteis, Laura de; Fuente, Jesús M. de la
Issue Date2019
Citation2nd Spanish Conference on Biomedical Applications of Nanomaterials (2019)
AbstractNowadays, in clinical trials, the complexity of the brain decreases the success rate of novel drugs against central nervous system diseases (CNDS). When using conventional drug delivery systems, a highly restrictive membrane minimizes the amount of drugs that reach the brain. The intranasal administration is an option to overpass this blood brain barrier in a non-invasive way to treat neurodegenerative illnesses, such as Alzheimer's disease. In this context, nanotechnology could greatly improve the efficacy of the therapies against CNDS through the design of nanocarriers, increasing the transport of the desired molecules through the nasal mucosa, their residence time and so the efficacy of the treatment. The optimal treatment would require that nanocarriers cross the nasal mucosa barrier, without losing their integrity or the active molecule. Therefore, it is important to define the fate of the carrier after administration characterizing the interaction between the nanocarrier and the biological environment, and deciphering if the release kinetics depend on the degradation of the whole carrier or the drug diffusion through it. In this work, nanocapsules (NCs), composed by an oily core and a chitosan shell, were synthesized for the encapsulation of two fluorophores, suitable for the appearance of Förster Resonance Energy Transfer (FRET) phenomenon, to monitor the nanocapsules integrity during in vitro tests. To take into account the interaction with the biological environment after administration, a simplified in vitro model of nasal mucosa was settled using Transwell (R) inserts and Calu-3 cells, an immortalized cell line derived from human adenocarcinoma of lungs, that preserves the characteristics of differentiated, functional human epithelia and is suitable as in vitro barrier model of the respiratory area. The developed material was tested in the in vitro model to study the interaction mechanism between nasal mucosa and chitosan-based NCs, for the evaluation of NCs fate and integrity.
DescriptionPóster presentado a la 2nd Spanish Conference on Biomedical Applications of Nanomaterials (SBAN), celebrada en Madrid del 6 al 7 de junio de 2019.
Appears in Collections:(ICMA) Comunicaciones congresos
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