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Application of the COST-Infogest static in vitro digestion model suitable for foods for the selection of robust probiotic candidates to be used in the ProInfant-CYTED project

AuthorsCarbonell, S.; Luaces, D.; Aznar, R.; Ruas-Madiedo, Patricia
Vegetable food
Gastrointestinal digestion
Issue Date2-Apr-2019
Citation6th International Conference on Food Digestion (2019)
AbstractIntroduction: The ProInfant-CYTED project, formed by a consortium of seven Ibero-American countries, aims the development of functional foods using native vegetables, obtained in a sustainable way, containing probiotics selected among the microbiota presents in local traditional fermented products. The best probiotic candidate, able to overproduce vitamins (riboflavin) and to counteract the activity of enteropathogens, will be evaluated in an intervention study with children from disadvantaged social strata in Guatemala. Objective: An adaptation of the sequential static digestion model suitable for foods, proposed by the COST Infogest network [Minekus et al, Food Funct. (2014) 5:1113], was used for the selection of the most in vitro robust probiotic candidates. Methodology: A collection of 36 LAB isolated from Andean foods was used to select 8 lactobacilli (Lactobacillus plantarum and Lactobacillus rhamnosus) according to health and safety criteria. These strains, and the probiotics 299V and GG, were included in a food matrix (65% corn, 15% soya, 20% skim-milk) or in saline solution; their survival was analysed following a modified procedure of the Infogest digestion model. Main finding: In general, the survival percentage of all strains after oral and gastric challenges was higher that 10% (reduction of one logarithmic unit) independently of the delivery carrier. After duodenal (10 minutes under intestinal fluid containing 10 mM bile and 100 units/mg porcine pancreatin) and intestinal challenges (2 h of the previous suspension diluted with 2 volumes of intestinal fluid) the viability dropped below 1%, with a reduction of more than 2 logarithmic units. Conclusion: Three L. plantarum (CECT8963, CECT9434, CECT9435) strains were selected because behaved better than the corresponding reference probiotic 299V. More than 2x107 CFU/ml were viable after this gastrointestinal simulation, which makes these strains good candidates to evaluate their functionality in more complex models.
DescriptionTrabajo presentado en el 6th International Conference on Food Digestion, celebrado en Granada (España), del 2 al 4 de abril de 2019
Appears in Collections:(IPLA) Comunicaciones congresos
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