English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/208558
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


The Microbiome of the Worldwide Invasive Ascidian Didemnum vexillum

AuthorsCasso, Maria ; Turon, Marta ; Marco, Núria; Pascual, Marta; Turon, Xavier
Introduced species
Vertical transmission
Horizontal transmission
Issue Date2020
PublisherFrontiers Media
CitationFrontiers in Marine Science 7 : 201 (2020)
AbstractAll multicellular organisms, including ascidians, host diverse microbial communities that are essential for their evolution. The global invader Didemnum vexillum is a colonial species native to Japan with two main genetic clades, A (the only invasive) and B, which provides a unique opportunity to assess if the microbiome remains stable in the colonization process or shifts according to local environment. We have analyzed, using 16S amplicon sequencing, the microbiome of 65 D. vexillum colonies from 13 populations worldwide including the two clades in the native area, plus samples from a congeneric species and seawater from one of the localities. We found 3,525 zeroradius operational taxonomic units (ZOTUs) in D. vexillum, belonging to 36 bacterial and 3 archaeal phyla. The microbiome of this species had a markedly different composition from surrounding seawater and from the congeneric species. For the globally invasive clade A, we found 3,154 ZOTUs, and 8 of them were present in all colonies, constituting a core microbiome with high abundance (69.57% of the total reads) but low diversity (0.25% of the total number of ZOTUs). The variable component was quantitatively much less important but comprised a highly diverse assemblage. In a multiple regression model, global microbiome structure correlated with differences in temperature range across localities and also with geographic distances, pointing to horizontal acquisition ofthesymbionts.However,theascidianmayhaveastrongcapacitytoselectandenrich itsmicrobiome,aswefoundthatthemostabundantZOTUsfromtunicsampleshadlow abundance in seawater samples from the same locality. The microbiome structure also correlated with the genetic distances between colonies obtained in a previous genomewide analysis, suggesting some potential for vertical transmission. In geographically restrictedcomparisons,temperatureandgeneticmakeup,butnotgeography,explained microbiome structure. The combination of a quantitatively dominant core component and a highly diverse variable fraction in the microbiome of D. vexillum can contribute to the success of this global invader in different environments.
DescriptionEste artículo contiene 13 páginas, 6 figuras, 3 tablas.
Publisher version (URL)https://doi.org/10.3389/fmars.2020.00201
Appears in Collections:(CEAB) Artículos
Files in This Item:
File Description SizeFormat 
Casso et al 2020 microbiome.pdf2,76 MBAdobe PDFThumbnail
Show full item record
Review this work

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.