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dc.contributor.authorDíaz Ramos, Margaritaes_ES
dc.contributor.authorPassos Miranda, Letíciaes_ES
dc.contributor.authorFernández-Lafuente, Robertoes_ES
dc.contributor.authorKopp, Williames_ES
dc.contributor.authorTardioli, Paulo Waldires_ES
dc.description.abstract[EN] Due to the heterogeneity of oils, the use of mixtures of lipases with di erent activity for a large number of glycerol-linked carboxylic acids that compose the substrate has been proposed as a better alternative than the use of one specific lipase preparation in the enzymatic synthesis of biodiesel. In this work, mixtures of lipases from di erent sources were evaluated in their soluble form in the ethanolysis of soybean oil. A mixture of lipases (50% of each lipase, in activity basis) from porcine pancreas (PPL) and Thermomyces lanuginosus lipase (TLL) gave the highest fatty acid ethyl ester (FAEE) yield (around 20 wt.%), while the individual lipases gave FAEE yields 100 and 5 times lower, respectively. These lipases were immobilized individually by the cross-linked enzyme aggregates (CLEAs) technique, yielding biocatalysts with 89 and 119% of expressed activity, respectively. A mixture of these CLEAs (also 50% of each lipase, in activity basis) gave 90.4 wt.% FAEE yield, while using separately CLEAs of PPL and TLL, the FAEE yields were 84.7 and 75.6 wt.%, respectively, under the same reaction conditions. The mixture of CLEAs could be reused (five cycles of 6 h) in the ethanolysis of soybean oil in a vortex flow-type reactor yielding an FAEE yield higher than 80% of that of the first batch.es_ES
dc.description.sponsorshipThis work was supported by the São Paulo Research Foundation (FAPESP) (grant numbers #2011/23194-0 and #2016/10636-8), Conselho Nacional Desenvolvimento Científico e Tecnológico (CNPq, grant numbers #405889/2016-0, #308212/2017-7), in part by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES)—Finance Code 001, and the MICIU of Spanish Government (project CTQ2017-86170-R).es_ES
dc.publisherMolecular Diversity Preservation Internationales_ES
dc.relation.isversionofPublisher's versiones_ES
dc.subjectporcine pancreas lipasees_ES
dc.subjectThermomyces lanuginosus lipasees_ES
dc.subjectMixture of CLEAses_ES
dc.subjectSoybean oil ethanolysises_ES
dc.subjectvortex flow reactores_ES
dc.titleImproving the Yields and Reaction Rate in the Ethanolysis of Soybean Oil by Using Mixtures of Lipase CLEAses_ES
dc.description.peerreviewedPeer reviewedes_ES
dc.rights.license© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license http://creativecommons.org/licenses/by/4.0/es_ES
dc.contributor.funderCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil)es_ES
dc.contributor.funderMinisterio de Ciencia y Tecnología (España)es_ES
dc.contributor.funderConselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil)es_ES
oprm.item.hasRevisionno ko 0 false*
dc.contributor.orcidFernández-Lafuente, Roberto [0000-0003-4976-7096]es_ES
dc.contributor.orcidTardioli, Paulo Waldir [0000-0002-5011-9881]es_ES
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