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Title

Improving the Yields and Reaction Rate in the Ethanolysis of Soybean Oil by Using Mixtures of Lipase CLEAs

AuthorsDíaz Ramos, Margarita; Passos Miranda, Letícia; Fernández-Lafuente, Roberto ; Kopp, William; Tardioli, Paulo Waldir
Keywordsporcine pancreas lipase
Thermomyces lanuginosus lipase
Mixture of CLEAs
Soybean oil ethanolysis
vortex flow reactor
Biodiesel
Issue Date1-Dec-2019
PublisherMolecular Diversity Preservation International
Abstract[EN] Due to the heterogeneity of oils, the use of mixtures of lipases with di erent activity for a large number of glycerol-linked carboxylic acids that compose the substrate has been proposed as a better alternative than the use of one specific lipase preparation in the enzymatic synthesis of biodiesel. In this work, mixtures of lipases from di erent sources were evaluated in their soluble form in the ethanolysis of soybean oil. A mixture of lipases (50% of each lipase, in activity basis) from porcine pancreas (PPL) and Thermomyces lanuginosus lipase (TLL) gave the highest fatty acid ethyl ester (FAEE) yield (around 20 wt.%), while the individual lipases gave FAEE yields 100 and 5 times lower, respectively. These lipases were immobilized individually by the cross-linked enzyme aggregates (CLEAs) technique, yielding biocatalysts with 89 and 119% of expressed activity, respectively. A mixture of these CLEAs (also 50% of each lipase, in activity basis) gave 90.4 wt.% FAEE yield, while using separately CLEAs of PPL and TLL, the FAEE yields were 84.7 and 75.6 wt.%, respectively, under the same reaction conditions. The mixture of CLEAs could be reused (five cycles of 6 h) in the ethanolysis of soybean oil in a vortex flow-type reactor yielding an FAEE yield higher than 80% of that of the first batch.
Publisher version (URL)http://dx.doi.org/10.3390/molecules24234392
URIhttp://hdl.handle.net/10261/208147
ISSN1420-3049
E-ISSN1420-3049
Appears in Collections:(ICP) Artículos
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