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Topological locking restrains replication fork reversal

AuthorsFierro-Fernández, Marta ; Hernandez, Pablo ; Krimer, Dora B. ; Stasiak, Andrzej Z.; Schvartzman, Jorge Bernardo
KeywordsDNA repair
DNA replication
DNA structure
DNA topology
DNA supercoiling
Issue Date30-Jan-2007
PublisherNational Academy of Sciences (U.S.)
CitationProc Natl Acad Sci 104(5):1500-5 (2007)
AbstractTwo-dimensional agarose gel electrophoresis, psoralen cross-linking, and electron microscopy were used to study the effects of positive supercoiling on fork reversal in isolated replication intermediates of bacterial DNA plasmids. The results obtained demonstrate that the formation of Holliday-like junctions at both forks of a replication bubble creates a topological constraint that prevents further regression of the forks. We propose that this topological locking of replication intermediates provides a biological safety mechanism that protects DNA molecules against extensive fork reversals.
Description6 p.-5 fig.
Publisher version (URL)https://dx.doi.org/10.1073/pnas.0609204104
Appears in Collections:(CIB) Artículos
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