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Molecular Characterization of Tc964, A Novel Antigenic Protein from Trypanosoma cruzi

AuthorsRuiz-Márvez, Elizabeth; Ramírez, César Augusto; Rodríguez, Eliana Rocío; Flórez, Magda Mellisa; Delgado, Gabriela; Guzmán, Fanny; Gómez-Puertas, Paulino ; Requena, José María ; Puerta, Concepción J.
Issue Date31-Mar-2020
PublisherMultidisciplinary Digital Publishing Institute
CitationInternational Journal of Molecular Sciences 21 (7): 2432 (2020)
AbstractThe Tc964 protein was initially identified by its presence in the interactome associated with the LYT1 mRNAs, which code for a virulence factor of <i>Trypanosoma cruzi</i>. Tc964 is annotated in the <i>T. cruzi</i> genome as a hypothetical protein. According to phylogenetic analysis, the protein is conserved in the different genera of the Trypanosomatidae family; however, recognizable orthologues were not identified in other groups of organisms. Therefore, as a first step, an in-depth molecular characterization of the Tc946 protein was carried out. Based on structural predictions and molecular dynamics studies, the Tc964 protein would belong to a particular class of GTPases. Subcellular fractionation analysis indicated that Tc964 is a nucleocytoplasmic protein. Additionally, the protein was expressed as a recombinant protein in order to analyze its antigenicity with sera from Chagas disease (CD) patients. Tc964 was found to be antigenic, and B-cell epitopes were mapped by the use of synthetic peptides. In parallel, the <i>Leishmania major</i> homologue (Lm964) was also expressed as recombinant protein and used for a preliminary evaluation of antigen cross-reactivity in CD patients. Interestingly, Tc964 was recognized by sera from Chronic CD (CCD) patients at different stages of disease severity, but no reactivity against this protein was observed when sera from Colombian patients with cutaneous leishmaniasis were analyzed. Therefore, Tc964 would be adequate for CD diagnosis in areas where both infections (CD and leishmaniasis) coexist, even though additional assays using larger collections of sera are needed in order to confirm its usefulness for differential serodiagnosis.
Identifiersdoi: 10.3390/ijms21072432
Appears in Collections:Colección MDPI
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