Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/20743
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Solé, Montserrat | - |
dc.contributor.author | Potrykus, J. | - |
dc.contributor.author | Fernández-Díez, Cristina | - |
dc.contributor.author | Blasco, Julián | - |
dc.date.accessioned | 2010-02-05T10:55:16Z | - |
dc.date.available | 2010-02-05T10:55:16Z | - |
dc.date.issued | 2004-03 | - |
dc.identifier.citation | Fish Physiology and Biochemistry 30(1): 57-66 (2004) | en_US |
dc.identifier.issn | 0920-1742 | - |
dc.identifier.uri | http://hdl.handle.net/10261/20743 | - |
dc.description | 10 pages, 4 figures, 2 tables | en_US |
dc.description.abstract | The biochemical status of antioxidant defences of larvae from the commercial, benthic fish, Solea senegalensis, were studied over a period of 28 days from hatching. The parameters studied were: catalase (EC 1.11.1.6), total glutation peroxidase activity (sum of the selenium dependent and independent forms) (EC 1.6.4.2), DT-diaphorase (EC 1.6.99.2), and as an indication of conjugative detoxifying metabolism, the enzyme glutathione S-transferase (GST; EC 2.5.1.18). Oxidative damage was evaluated by the formation of malondialdehyde (MDA) as an indication of lipid peroxidation (LP). Metallothionein (MT) levels were measured by differential pulse polarography and stress proteins (HSP 70 and HSP 60) were detected by immunoblotting, using commercial antibodies. The presence of catalytic activities was observed from hatching day and tended to increase during larval development. Significant changes were seen in most enzymes at day 3, when the larvae finished their endogenous feeding and started to feed on rotifers. DT-diaphorase activities were similar in both NADH and NADPH dependent forms and, in turn, were about 10-times lower than their correspondent reductases. LP sharply increased at 19 and 28 days post-hatch (dph), suggesting a saturation of the antioxidant defences during metamorphosis. MT showed high values during the endogenous phase and the lowest value corresponded to day 3, when the egg-yolksac was fully reabsorbed; a steady-state value was reached thereafter. Stress proteins were present at all stages, showing distinctiveness in the molecular weight of HSP60 at hatching day and 1 dph. Quantitatively, both forms were significantly elevated at 3 dph, with their ratio (HSP70/HSP60) decreasing over time | en_US |
dc.description.sponsorship | This work was partially funded by a CICYT project (AGL-2003-03558). The authors are grateful for the visiting grant to Dr J. Potrykus from a CSIC-PAN collaboration and to Dr J.P. Can˜ avate from CIFPA ‘‘El Torun˜ o’’ for providing the fish egg stock | en_US |
dc.format.extent | 22195 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | en_US |
dc.publisher | Springer Nature | en_US |
dc.rights | closedAccess | en_US |
dc.subject | Antioxidant enzymes | en_US |
dc.subject | Fish larvae | en_US |
dc.subject | Lipid peroxidation | en_US |
dc.subject | Metallothionein | en_US |
dc.subject | ROS | en_US |
dc.subject | Solea senegalensis | en_US |
dc.subject | Stress proteins | en_US |
dc.title | Variations on stress defences and metallothionein levels in the Senegal sole, Solea senegalensis, during early larval stages | en_US |
dc.type | artículo | en_US |
dc.identifier.doi | 10.1007/s10695-004-6786-6 | - |
dc.description.peerreviewed | Peer reviewed | en_US |
dc.relation.publisherversion | https://doi.org/10.1007/s10695-004-6786-6 | en_US |
dc.identifier.e-issn | 1573-5168 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | No Fulltext | - |
item.languageiso639-1 | en | - |
Aparece en las colecciones: | (ICM) Artículos (ICMAN) Artículos |
CORE Recommender
SCOPUSTM
Citations
58
checked on 24-abr-2024
WEB OF SCIENCETM
Citations
53
checked on 27-feb-2024
Page view(s)
400
checked on 23-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.