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Model-guided construction of recombinant S. cerevisiae strains showing lower acetate yield under aerobic conditions

AuthorsRodrigues, Alda Joao; Andrejev; Andrejev, S.; Jouhten, Paula; Patil, Kiran R.; Tronchoni, Jordi ; Morales, Pilar ; González García, Ramón
Issue Date24-Jun-2019
Citation7th Conference on Physiology of Yeast and Filamentous (2019)
AbstractExcess acetate yield under aerobiosis is a major drawback for S. cerevisiae and some other yeast species to be used in winemaking processes aiming to reduce ethanol content of wine (1). Yet, the biochemical mechanisms underlying the observed excess acetate production under aerobic conditions are unclear. We therefore used a genomic-scale modelling approach, combined with experimentally determined uptake/secretion rates, to gain insights into the metabolic reorganization in S. cerevisiae in response to oxygen under conditions relevant for winemaking. The model was also used to identify gene deletion targets towards altering the production of acetic acid in the presence of oxygen. Several gene knock-out targets with expected reduction in acetic acid production were thus obtained using for MoMa and MiMBl algorithms. The model predictions were tested in the fermentation of natural grape must under aerobic conditions with strains from the prototrophic yeast knockout collection. Although many of the prediction did not show any reduction in acetic acid yield, and increased yield was observed in some cases, acetate reduction was obtained for three gene deletion strains, ald6¿, mdh3¿ and rip1¿. The involvement of ALD6 on aerobic acetic acid production could be expected from published results. The results for MDH3 and RIP1 could not be easily anticipated and thus provide new insights into yeast metabolism concerning acetate production.
DescriptionTrabajo presentado en la 7th Conference on Physiology of Yeast and Filamentous, celebrado en Milán (Italia), del 24 al 27 de junio de 2019
Appears in Collections:(ICVV) Comunicaciones congresos
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