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Title

Identification of target genes to reduce acetate yield by Saccharomyces cerevisiae under aerobic conditions

AuthorsRodrigues, Alda Joao; Andrejev; Andrejev, S.; Jouhten, Paula; Patil, Kiran R.; Tronchoni, Jordi ; Morales, Pilar ; González García, Ramón
Issue Date10-Jun-2019
CitationBIOTEC (2019)
AbstractExcessive acetic acid production is a key problem in winemaking processes aiming to reduce ethanol content of wine (1). It is related to a boost in acetate yields observed for Saccharomyces cerevisiae and some other yeasts under aerobic fermentation conditions. However, the biochemical mechanisms underlying this problem are unclear. We therefore used a genomic-scale modelling approach, combined with experimentally determined uptake/secretion rates, to gain insights into the metabolic rewiring of S. cerevisiae in response to oxygen under conditions relevant for winemaking. The model was also used to identify gene deletion targets affecting the production of acetic acid under aerobic conditions. Several gene knock-out with expected reduction in acetic acid production were thus identified as targets using MoMa and MiMBl algorithms. The predictions were tested using strains from the prototrophic yeast knockout collection for the fermentation of natural grape must under aerobic conditions. Although many of the prediction did not show any reduction in acetic acid yield, and increased yield was observed in some cases, significant acetate reduction was obtained for three gene deletion strains, ald6¿, mdh3¿ and rip1¿. The involvement of ALD6 on aerobic acetic acid production could be expected from results by other authors. However, the results for MDH3 and RIP1 could not be easily anticipated and thus provide new insights into yeast metabolism concerning acetic acid production, a major concern for the wine industry.
DescriptionTrabajo presentado en Congreso Nacional de Biotecnología (BIOTEC 2019), celebrado en Vigo (España), del 10 al 13 de junio de 2019
URIhttp://hdl.handle.net/10261/207323
Appears in Collections:(ICVV) Comunicaciones congresos
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