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Title

HC-Pro hypo- and hypersuppressor mutants: differences in viral siRNA accumulation in vivo and siRNA binding activity in vitro

AuthorsTorres-Barceló, Clara ; Daròs Arnau, José Antonio ; Elena, Santiago F. ; Elena, Santiago F.
Keywordsvirus evolution
TEV
suppression
RNA silencing
experimental evolution
RNA binding
Issue Date2-Feb-2010
PublisherSpringer
CitationArchives of Virology, 155: 251-4 (2010)
Series155: 251-254
AbstractViruses have evolved mechanisms to suppress the RNA silencing defense of their hosts, allowing replication and systemic colonization. In a recent study, we found that the effect of mutations in the RNA silencing suppressor of Tobacco etch virus (TEV) was variable, ranging from complete abolition of suppressor activity to significantly stronger suppression. Whereas hyposuppressor mutants were less virulent and accumulated less viral particles than wildtype, hypersuppressors induced symptoms similar to wildtype and accumulated to similar levels. Here we further characterize a set of these mutants in terms of their ability to bind in vitro and induce accumulation in vivo of virus-derived siRNAs. Hyposuppressor alleles are less efficient binding siRNAs than hypersuppressors, whereas the latter are not different from the wildtype. As a consequence of lower viral accumulation, plants infected with virus bearing a hyposuppressor allele also accumulate less virus-derived siRNAs.
Publisher version (URL)http://www.springerlink.com/content/u756t833076092u5/fulltext.html
URIhttp://hdl.handle.net/10261/20674
DOI10.1007/s0076-009-0563-x
Appears in Collections:(IBMCP) Artículos
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