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Search for proteomic biomarkers related to bovine pre-slaughter stress using liquid isoelectric focusing (OFFGEL) and mass spectrometry

AuthorsFuente García, Claudia; Aldai, Noelia CSIC ORCID; Sentandreu, Enrique CSIC ORCID; Oliván, Mamen; García-Torres, Susana; Franco, Daniel; Zapata, Carlos; Sentandreu, Miguel Angel CSIC ORCID
KeywordsDFD meat
Pre-slaughter stress
Protein biomarkers
Mass spectrometry
Issue Date29-Oct-2018
CitationJournal of Proteomics 198: 59-65 (2019)
AbstractProteome changes derived from animals that have suffered pre-slaughter stress are a fact. In this study, Proteomic analysis was carried out on 20 bovine loin samples from Asturiana de los Valles and crossbreds cattle previously classified as normal and DFD meat at 24 h post-mortem using pH measurements. Sarcoplasmic sub-proteome of Longissimus thoracis at 24 h post-mortem was fractionated by the use of liquid isoelectric focusing (OFFGEL) in the pH range 3–10, followed by SDS-PAGE analysis of each retrieved fraction. The protein fractionation profile showed high reproducibility along the different sample groups. Five protein bands showed significant differences (p < 0.05) between the two groups, allowing discrimination between them. Proteins present in these bands, which were identified by LC-MS, were actin, phosphoglucomutase-1, alpha-crystallin B, heat shock protein beta-6 and heat shock protein beta-1.
Publisher version (URL)https://doi.org/10.1016/j.jprot.2018.10.013
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