English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/206376
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


An empirical assessment of a single family-wide hybrid capture locus set at multiple evolutionary timescales in Asteraceae

AuthorsJones, Katy E.; Fér, Tomáš; Schmickl, Roswitha E.; Dikow, Rebecca B.; Funk, Vicki A.; Herrando Moraira, Sonia ; Kilian, Norbert; Siniscalchi, Carolina M.; Susanna de la Serna, Alfonso ; Slovák, Marek; Thapa, Ramhari; Watson, Linda; Mandel, Jennifer R.
Hybrid capture
Non-paralogy phylogenetics.
Issue DateOct-2019
PublisherJohn Wiley & Sons
CitationApplications in Plant Sciences 7(10): e11295 (2019)
Abstract[Premise] Hybrid capture with high‐throughput sequencing (Hyb‐Seq) is a powerful tool for evolutionary studies. The applicability of an Asteraceae family‐specific Hyb‐Seq probe set and the outcomes of different phylogenetic analyses are investigated here.[ [Methods] Hyb‐Seq data from 112 Asteraceae samples were organized into groups at different taxonomic levels (tribe, genus, and species). For each group, data sets of non‐paralogous loci were built and proportions of parsimony informative characters estimated. The impacts of analyzing alternative data sets, removing long branches, and type of analysis on tree resolution and inferred topologies were investigated in tribe Cichorieae. [Results] Alignments of the Asteraceae family‐wide Hyb‐Seq locus set were parsimony informative at all taxonomic levels. Levels of resolution and topologies inferred at shallower nodes differed depending on the locus data set and the type of analysis, and were affected by the presence of long branches. [Discussion] The approach used to build a Hyb‐Seq locus data set influenced resolution and topologies inferred in phylogenetic analyses. Removal of long branches improved the reliability of topological inferences in maximum likelihood analyses. The Astereaceae Hyb‐Seq probe set is applicable at multiple taxonomic depths, which demonstrates that probe sets do not necessarily need to be lineage‐specific.
Publisher version (URL)http://dx.doi.org/10.1002/aps3.11295
Identifiersdoi: 10.1002/aps3.11295
issn: 2168-0450
Appears in Collections:(IBB) Artículos
Files in This Item:
File Description SizeFormat 
Empirical_assessment.pdf1,37 MBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.