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Título: | Use of cavity ring-down spectrometry to quantify 13 C-primary productivity in oligotrophic waters |
Autor: | López-Sandoval, Daffne CSIC ORCID; Delgado Huertas, Antonio CSIC ORCID ; Carrillo de Albornoz, Paloma; Duarte, Carlos M. CSIC ORCID; Agustí, Susana CSIC ORCID | Fecha de publicación: | 19-ene-2019 | Editor: | American Society of Limnology and Oceanography | Citación: | Limnology and Oceanography: Methods 17: 137-144 (2019) | Resumen: | Cavity ring-down spectroscopy (CRDS) is a highly sensitive laser technique that allows the analysis ofisotopic signals and absolute concentration of individual molecular species in small-volume samples. Here, wedescribe a protocol to quantify photosynthetic13C-uptake rates of marine phytoplankton by using the CRDStechnique (13C-CRDS-PP). We validated our method by comparing the13C-PP rates measured between CRDSand isotope ratio mass spectrometry (IRMS) in samples with different carbon content (30–160μgC). The com-parison revealed that13C-CRDS-PP rates were highly correlated with those obtained by IRMS (Spearman correla-tion coefficient,ρ= 0.95,p< 0.0001,n= 15), with a mean difference between the two estimates of0.08 mgCm−3h−1. Moreover, the slope of the relationship between CRDS and IRMS results was not significantly differentfrom 1 (F= 0.03,p= 0.86), and the intercept did not differ from 0 (F= 1.4,p= 0.24), indicating that there wasno bias in the CRDS relative to the IRMS-based measurements. A separate analysis also showed that despite thedifference in volume and carbon content between samples (4010μgC and 16040μgC, respectively), the13C-CRDS-PP technique provides similar results (Mann–Whitney test,U= 30.5,p= 0.90,n= 8). In addition,13C-CRDS-PP rates measured along the Red Sea (176 mgC m−2d−1) agreed with14C-based PP rates previouslyreported for similar locations. Thus, this study evidenced that the13C-CRDS-PP method is sensitive enough toquantify carbonfixation rates in oligotrophic regions. | Versión del editor: | http://dx.doi.org/10.1002/lom3.10305 | URI: | http://hdl.handle.net/10261/206123 | DOI: | 10.1002/lom3.10305 | Identificadores: | doi: 10.1002/lom3.10305 issn: 1541-5856 |
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