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dc.contributor.authorLado, Joannaes_ES
dc.contributor.authorZacarías, Jaimees_ES
dc.contributor.authorRodrigo, María Jesúses_ES
dc.contributor.authorZacarías, Lorenzoes_ES
dc.date.accessioned2020-03-31T13:38:01Z-
dc.date.available2020-03-31T13:38:01Z-
dc.date.issued2019-11-20-
dc.identifier.citationMethods in Molecular Biology 2083: 235-244 (2020)es_ES
dc.identifier.issn1064-3745-
dc.identifier.urihttp://hdl.handle.net/10261/206047-
dc.description.abstractPlastids are cell organelles that, beside other functions, have the capability to store carotenoids in specialized structures, which may vary among the different plant species, tissues or according to the carotenoid complement. Fruits are an important source of carotenoids, and during ripening, chloroplasts differentiate into chromoplasts that are able to accumulate large amounts of carotenoids, rendering then the characteristic fruit coloration. Whereas lycopene or β-carotene may accumulate as crystal in the chromoplasts of some fruit, other xanthophyll-accumulating fruits differentiate plastoglobuli as a preferred system to enhance carotenoids stability and storage. Visualization of plastid ultrastructure and their transformation during ripening or in fruit of contrasting coloration are fundamental objectives within carotenoids research in fruits. Therefore, in this chapter, we describe a protocol for the visualization and analysis of plastid ultrastructure by transmission electron microscopy (TEM), specially designed and adapted to fruit tissues.es_ES
dc.description.sponsorshipFinancial support by research grants AGL-2015-70218 (Ministerio Economía y Competitividad (Spain), and FEDEGENT/2018/007 (Generalitat Valenciana, Spain) are gratefully acknowledged. MJR and LZ are members of Eurocaroten (COST_Action CA15136) and CaRed (Spanish Carotenoid Network, BIO2015-71703-REDT and BIO2017-90877-REDT).es_ES
dc.language.isoenges_ES
dc.publisherHumana Presses_ES
dc.relationMINECO/ICTI2013-2016/AGL2015-70218-Res_ES
dc.relation.isversionofPostprintes_ES
dc.rightsembargoedAccesses_ES
dc.subjectCarotenoidses_ES
dc.subjectCitrus fruitses_ES
dc.subjectChloroplastes_ES
dc.subjectChromoplastes_ES
dc.subjectPlastidses_ES
dc.subjectPlastoglobulies_ES
dc.subjectTransmission electron microscopyes_ES
dc.titleVisualization of Carotenoid-Storage Structures in Fruits by Transmission Electron Microscopyes_ES
dc.typeartículoes_ES
dc.identifier.doi10.1007/978-1-4939-9952-1_18-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.1007/978-1-4939-9952-1_18es_ES
dc.embargo.terms2020-11-20es_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.contributor.funderGeneralitat Valencianaes_ES
dc.contributor.funderEuropean Commissiones_ES
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000780es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003359es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
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