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Title

Interferon-β Stimulation Elicited by the Influenza Virus Is Regulated by the Histone Methylase Dot1L through the RIG-I-TRIM25 Signaling Axis

AuthorsMarcos-Villar, Laura CSIC ORCID ; Nistal-Villán, Estanislao; Zamarreño, Noelia; Garaigorta, Urtzi; Gastaminza, Pablo; Nieto, Amelia
KeywordsDot1L histone methylase
TRIM25
Influenza virus
RIG-I signaling
Type I IFN
RNA virus infection
Issue Date2020
PublisherMultidisciplinary Digital Publishing Institute
CitationCells 9(3): 732 (2020)
AbstractInfluenza virus infection increases the methylation of lysine 79 of histone 3 catalyzed by the Dot1L enzyme. The role of Dot1L against infections was highlighted by an increase of influenza A and vesicular stomatitis virus replication in Dot1L-inhibited cells mediated by a decreased antiviral response. Interferon-beta (IFN-β) reporter assays indicate that Dot1L is involved in the control of retinoic acid-inducible gene-I protein (RIG-I) signaling. Accordingly, Dot1L inhibition decreases the IFN-β promoter stimulation and RIG-I- mitochondria-associated viral sensor (RIG-I-MAVS) association upon viral infection. Replication of an influenza A virus lacking NS1 (delNS1), incapable of counteracting the antiviral response, is not affected by Dot1L inhibition. Consequently, RIG-I-MAVS association and nuclear factor–κB (NF-κB) nuclear translocation, are not affected by the Dot1L inhibition in delNS1 infected cells. Restoration of NS1 expression in trans also reinstated Dot1L as a regulator of the RIG-I-dependent signaling in delNS1 infections. Interferon-inducible E3 ligase tripartite motif-containing protein 25 (TRIM25) expression increases in influenza virus infected cells, but Dot1L inhibition reduces both the TRIM25 expression and TRIM25 protein levels. TRIM25 overexpression reverses the defective innate response mediated by Dot1L inhibition elicited upon virus infection or by overexpression of RIG-I signaling intermediates. Thus, TRIM25 is a control point of the RIG-I recognition pathway controlled by Dot1L and may have a general role in RNA viruses recognized by the RIG-I sensor.
Description© 2020 by the authors.
Publisher version (URL)https://doi.org/10.3390/cells9030732
URIhttp://hdl.handle.net/10261/205581
DOI10.3390/cells9030732
E-ISSN2073-4409
Appears in Collections:(CNB) Artículos

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