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Acquisition of Mass Spectrometry Data of Carotenoids: A Focus on Big Data Management

AuthorsPérez Gálvez, Antonio CSIC ORCID ; Viera Alcaide, Isabel CSIC ORCID ; Roca, María CSIC ORCID
Issue Date2020
CitationMethods in molecular biology (Clifton, N.J.) 2083: 135- 144 (2020)
AbstractAccurate determination of carotenoid profile in plant tissues and food samples requires the application of hyphenated analytical resources including HPLC with high-resolution hybrid mass spectrometers. The high analytical power of modern MS equipment means the generation of Big Data resulting from the independent and complementary physicochemical properties of the target compounds that requires a complex processing to unravel the results. The present protocol describes a complete pipeline methodology for high-throughput analysis of carotenoids based on mass spectrometry (MS). After an exhaustive extraction, the workflow includes the acquisition of HPLC-hr-MS and MS2 spectra assisted step by step by specific post-processing software.
DescriptionPérez-Gálvez A., Viera I., Roca M. (2020) Acquisition of Mass Spectrometry Data of Carotenoids: A Focus on Big Data Management. In: Rodríguez-Concepción M., Welsch R. (eds) Plant and Food Carotenoids. Methods in Molecular Biology, vol 2083. Humana, New York, NY
Publisher version (URL)http://dx.doi.org/10.1007/978-1-4939-9952-1_10
Identifiersdoi: 10.1007/978-1-4939-9952-1_10
issn: 1940-6029
isbn: 978-1-4939-9951-4
Appears in Collections:(IG) Libros y partes de libros
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