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dc.contributor.authorContreras Montoya, Rafael-
dc.contributor.authorCastellví, Albert-
dc.contributor.authorEscolano-Casado, Guillermo-
dc.contributor.authorJuanhuix, Judith-
dc.contributor.authorConejero Muriel, Mayte-
dc.contributor.authorLópez López, Modesto-
dc.contributor.authorCuerva, Juan M.-
dc.contributor.authorÁlvarez de Cienfuegos, Luis-
dc.contributor.authorGavira, José A.-
dc.date.accessioned2020-03-26T08:05:23Z-
dc.date.available2020-03-26T08:05:23Z-
dc.date.issued2019-05-21-
dc.identifierdoi: 10.1021/acs.cgd.9b00131-
dc.identifierissn: 1528-7505-
dc.identifier.citationCrystal Growth and Design 19 (8): 4229- 4233 (2019)-
dc.identifier.urihttp://hdl.handle.net/10261/205205-
dc.description.abstractLysozyme crystals grown in Fmoc-CF (Cys-Phe) hydrogels, unlike those grown in agarose, give rise to composite crystals that have an enhanced resistance against degradation caused by an intense exposure to X-ray irradiation. Fmoc-CF dipeptide shows a clear protection of the most sensitive groups (disulfide bonds and methionines) of the protein. The protection mediated by cysteine is exerted only in its gel state since cysteine in solution has an adverse effect. Probably, the reactive thiol groups of cysteine being locked within the rigid peptide fibers minimize cross-reactions with the proteins favoring the formation of protein crystals. Once located inside the protein crystal, the long peptide fibers are able to protect the protein against radiation damage. ©-
dc.description.sponsorshipThis study was supported by projects BIO2016−74875-P (JAG) and FIS2017−85954-R (LAC) (Ministerio de Economía, Industria y Competitividad, MINECO, and Agencia Estatal de Investigación, AEI, Spain, cofunded by Fondo Europeo de Desarrollo Regional, FEDER, European Union) and by Junta de Andalucía (Spain) projects P12-FQM-2721 and P12-FQM-790-
dc.languageeng-
dc.publisherAmerican Chemical Society-
dc.relationMINECO/ICTI2013-2016/BIO2016-74875-P-
dc.relationMINECO/ICTI2013-2016/FIS2017-85954-R-
dc.relation.isversionofPublisher's version-
dc.rightsclosedAccess-
dc.titleEnhanced Stability against Radiation Damage of Lysozyme Crystals Grown in Fmoc-CF Hydrogels-
dc.typeartículo-
dc.identifier.doi10.1021/acs.cgd.9b00131-
dc.relation.publisherversionhttp://dx.doi.org/10.1021/acs.cgd.9b00131-
dc.date.updated2020-03-26T08:05:23Z-
dc.contributor.funderMinisterio de Economía, Industria y Competitividad (España)-
dc.contributor.funderAgencia Estatal de Investigación (España)-
dc.contributor.funderEuropean Commission-
dc.contributor.funderJunta de Andalucía-
dc.relation.csic-
dc.identifier.funderhttp://dx.doi.org/10.13039/501100011033es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000780es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100010198es_ES
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