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Diagnosis of Albendazole Resistance in Fasciola Hepatica

AuthorsAlvarez, L.; Ceballos, L.; Canton, C.; Pruzzo, C.; Sanabria, R.; Moreno, L.; Sanchis, J.; Ortiz, P.; Fairweather, I.; Lanusse, C.; Martínez Valladares, María CSIC ORCID CVN
Issue Date7-Jul-2019
Citation27th International Conference of the World Association for the Advancement of Veterinary Parasitology: 128 (2019)
AbstractThe increasing widespread development of drug resistance in the liver fluke Fasciola hepatica has motivated the need for alternative diagnostic tools. The work reported here describes the validation of an egg hatch test (EHT) as an in vitro technique to detect albendazole (ABZ) resistance in F. hepatica. The validation includes the intraassay, inter-assay and intra-herd variations, and the comparison of results obtained after performing the EHT and a controlled eicacy test. Additionally, the development of the protocol included the adjustment of di@erent critical factors to improve the simplicity of the assay. The greatest uniformity between results within the assay and over time until 8 weeks after gallbladder eggs collection (the deadline proposed for egg analysis), was obtained after incubation with an ABZ concentration of 0.5 µM. The length of exposure to ABZ was shown to be critical, as prolonged (15 days) ABZ incubation led to a reversal of drug resistance. There was a close agreement between the outcome of the EHT and that obtained for the in vivo assays. Moreover, the same level of resistance was observed when eggs and faeces were collected from animals of four (4) di@erent farms and analyzed with both the EHT and the faecal egg count reduction test. A 0.5 µM drug level is confirmed as the discriminating concentration to predict ABZ resistance by the EHT in F. hepatica.
DescriptionTrabajo presentado al: 27th International Conference of the World Association for the Advancement of Veterinary Parasitology. Abstract book, Oral Session OA 35.01, pp. 128. Madison (USA), 7-11 julio 2019.
Appears in Collections:(IGM) Comunicaciones congresos
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