Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/204782
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dc.contributor.authorAlmazán, Fernando-
dc.contributor.authorSolá Gurpegui, Isabel-
dc.contributor.authorZúñiga Lucas, Sonia-
dc.contributor.authorMárquez-Jurado, Silvia-
dc.contributor.authorMorales, Lucía-
dc.contributor.authorBecares, Martina-
dc.contributor.authorEnjuanes Sánchez, Luis-
dc.date.accessioned2020-03-23T09:59:30Z-
dc.date.available2020-03-23T09:59:30Z-
dc.date.issued2014-08-30-
dc.identifierdoi: 10.1016/j.virusres.2014.05.026-
dc.identifierissn: 0168-1702-
dc.identifier.citationVirus Research 189: 262-270 (2014)-
dc.identifier.urihttp://hdl.handle.net/10261/204782-
dc.description.abstractCoronaviruses (CoVs) infect humans and many animal species, and are associated with respiratory, enteric, hepatic, and central nervous system diseases. The large size of the CoV genome and the instability of some CoV replicase gene sequences during its propagation in bacteria, represent serious obstacles for the development of reverse genetic systems similar to those used for smaller positive sense RNA viruses. To overcome these limitations, several alternatives to more conventional plasmid-based approaches have been established in the last 13 years. In this report, we briefly review and discuss the different reverse genetic systems developed for CoVs, paying special attention to the severe acute respiratory syndrome CoV (SARS-CoV).-
dc.description.sponsorshipThis work was supported by grants from the Ministry of Science and Innovation of Spain (MCINN) (BIO2010-16705), the European Community’s Seventh Framework Programme (FP7/2007–2013) under the project “EMPERIE” (HEALTH-F3-2009-223498), and the National Institute of Health (NIH) of USA (2P01AI060699-06A1).-
dc.languageeng-
dc.publisherElsevier BV-
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/223498-
dc.relation.isversionofPublisher’s version-
dc.rightsopenAccess-
dc.subjectCoronavirus-
dc.subjectReverse genetics-
dc.subjectInfectious clones-
dc.subjectReplicons-
dc.titleCoronavirus reverse genetic systems: Infectious clones and replicons-
dc.typeartículo-
dc.identifier.doi10.1016/j.virusres.2014.05.026-
dc.relation.publisherversionhttp://dx.doi.org/10.1016/j.virusres.2014.05.026-
dc.date.updated2020-03-23T09:59:31Z-
dc.contributor.funderMinisterio de Ciencia e Innovación (España)-
dc.contributor.funderEuropean Commission-
dc.contributor.funderNational Institutes of Health (US)-
dc.relation.csic-
dc.identifier.funderhttp://dx.doi.org/10.13039/100000002es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100004837es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000780es_ES
dc.identifier.pmid24930446-
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.grantfulltextopen-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.fulltextWith Fulltext-
item.openairetypeartículo-
Appears in Collections:(VICYT) Colección Especial COVID-19
(CNB) Artículos
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