Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/204782
Share/Export:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Title

Coronavirus reverse genetic systems: Infectious clones and replicons

AuthorsAlmazán, Fernando CSIC ORCID ; Solá Gurpegui, Isabel ; Zúñiga Lucas, Sonia CSIC ORCID ; Márquez-Jurado, Silvia CSIC ORCID ; Morales, Lucía CSIC ORCID ; Becares, Martina ; Enjuanes Sánchez, Luis CSIC ORCID
KeywordsCoronavirus
Reverse genetics
Infectious clones
Replicons
Issue Date30-Aug-2014
PublisherElsevier BV
CitationVirus Research 189: 262-270 (2014)
AbstractCoronaviruses (CoVs) infect humans and many animal species, and are associated with respiratory, enteric, hepatic, and central nervous system diseases. The large size of the CoV genome and the instability of some CoV replicase gene sequences during its propagation in bacteria, represent serious obstacles for the development of reverse genetic systems similar to those used for smaller positive sense RNA viruses. To overcome these limitations, several alternatives to more conventional plasmid-based approaches have been established in the last 13 years. In this report, we briefly review and discuss the different reverse genetic systems developed for CoVs, paying special attention to the severe acute respiratory syndrome CoV (SARS-CoV).
Publisher version (URL)http://dx.doi.org/10.1016/j.virusres.2014.05.026
URIhttp://hdl.handle.net/10261/204782
DOI10.1016/j.virusres.2014.05.026
Identifiersdoi: 10.1016/j.virusres.2014.05.026
issn: 0168-1702
Appears in Collections:(VICYT) Colección Especial COVID-19
(CNB) Artículos

Files in This Item:
File Description SizeFormat
Coronavirus reverse_Almazan.pdf1,18 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work

PubMed Central
Citations

53
checked on Jan 16, 2022

SCOPUSTM   
Citations

67
checked on Jan 20, 2022

WEB OF SCIENCETM
Citations

60
checked on Jan 16, 2022

Page view(s)

206
checked on Jan 21, 2022

Download(s)

332
checked on Jan 21, 2022

Google ScholarTM

Check

Altmetric

Dimensions


Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.