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dc.contributor.authorAmado, Eric-
dc.contributor.authorMuth, Günther-
dc.contributor.authorArechaga, Ignacio-
dc.contributor.authorCabezón, Elena-
dc.date.accessioned2020-03-20T13:47:46Z-
dc.date.available2020-03-20T13:47:46Z-
dc.date.issued2019-02-05-
dc.identifier.citationJournal of Biological Chemistry 294: 5050-5059 (2019)-
dc.identifier.issn0021-9258-
dc.identifier.urihttp://hdl.handle.net/10261/204685-
dc.description.abstractTraB is an FtsK-like DNA translocase responsible for conjugative plasmid transfer in mycelial Streptomyces. Unlike other conjugative systems, which depend on a type IV secretion system, Streptomyces requires only TraB protein to transfer the plasmid as dsDNA. The γ-domain of this protein specifically binds to repeated 8-bp motifs on the plasmid sequence, following a mechanism that is reminiscent of the FtsK/SpoIIIE chromosome segregation system. In this work, we purified and characterized the enzymatic activity of TraB, revealing that it is a DNA-dependent ATPase that is highly stimulated by dsDNA substrates. Interestingly, we found that unlike the SpoIIIE protein, the γ-domain of TraB does not confer sequence-specific ATPase stimulation. We also found that TraB binds G-quadruplex DNA structures with higher affinity than TraB-recognition sequences (TRSs). An EM-based structural analysis revealed that TraB tends to assemble as large complexes comprising four TraB hexamers, which might be a prerequisite for DNA translocation across cell membranes. In summary, our findings shed light on the molecular mechanism used by the DNA-translocating motor TraB, which may be shared by other membrane-associated machineries involved in DNA binding and translocation.-
dc.description.sponsorshipThis work was supported by the Spanish Ministerio de Economia y Competitividad (MINECO) grants BFU2016-78521-R (to E.C. and I.A.) and Deutsche Forschungsgemeinschaft SFB766 (to G.M).-
dc.languageeng-
dc.publisherAmerican Society for Biochemistry and Molecular Biology-
dc.relationMINECO/ICTI2013-2016/BFU2016-78521-R-
dc.rightsopenAccess-
dc.subjectStreptomyces-
dc.subjectBacterial conjugation-
dc.subjectDNA segregation-
dc.subjectDNA translocase-
dc.subjectFtsK-like ATPase-
dc.subjectDNA-dependent ATPas-
dc.subjectConjugal plasmid transfer-
dc.subjectMotor protein-
dc.subjectTRS sequence-
dc.titleThe FtsK-like motor TraB is a DNA-dependent ATPase that forms higher-order assemblies-
dc.typeartículo-
dc.identifier.doi10.1074/jbc.RA119.007459-
dc.relation.publisherversionhttp://dx.doi.org/10.1074/jbc.RA119.007459-
dc.identifier.e-issn1083-351X-
dc.date.updated2020-03-20T13:47:47Z-
dc.contributor.funderMinisterio de Economía y Competitividad (España)-
dc.contributor.funderGerman Research Foundation-
dc.relation.csic-
dc.identifier.funderhttp://dx.doi.org/10.13039/501100001659es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
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