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Title

The FtsK-like motor TraB is a DNA-dependent ATPase that forms higher-order assemblies

AuthorsAmado, Eric; Muth, Günther; Arechaga, Ignacio ; Cabezón, Elena
KeywordsStreptomyces
Bacterial conjugation
DNA segregation
DNA translocase
FtsK-like ATPase
DNA-dependent ATPas
Conjugal plasmid transfer
Motor protein
TRS sequence
Issue Date5-Feb-2019
PublisherAmerican Society for Biochemistry and Molecular Biology
CitationJournal of Biological Chemistry 294: 5050-5059 (2019)
AbstractTraB is an FtsK-like DNA translocase responsible for conjugative plasmid transfer in mycelial Streptomyces. Unlike other conjugative systems, which depend on a type IV secretion system, Streptomyces requires only TraB protein to transfer the plasmid as dsDNA. The γ-domain of this protein specifically binds to repeated 8-bp motifs on the plasmid sequence, following a mechanism that is reminiscent of the FtsK/SpoIIIE chromosome segregation system. In this work, we purified and characterized the enzymatic activity of TraB, revealing that it is a DNA-dependent ATPase that is highly stimulated by dsDNA substrates. Interestingly, we found that unlike the SpoIIIE protein, the γ-domain of TraB does not confer sequence-specific ATPase stimulation. We also found that TraB binds G-quadruplex DNA structures with higher affinity than TraB-recognition sequences (TRSs). An EM-based structural analysis revealed that TraB tends to assemble as large complexes comprising four TraB hexamers, which might be a prerequisite for DNA translocation across cell membranes. In summary, our findings shed light on the molecular mechanism used by the DNA-translocating motor TraB, which may be shared by other membrane-associated machineries involved in DNA binding and translocation.
Publisher version (URL)http://dx.doi.org/10.1074/jbc.RA119.007459
URIhttp://hdl.handle.net/10261/204685
DOI10.1074/jbc.RA119.007459
ISSN0021-9258
E-ISSN1083-351X
Appears in Collections:(IBBTEC) Artículos
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