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Transcriptome assembly and polymorphism detection in Silene ciliata (Caryophyllaceae)

AuthorsSacristán-Bajo, Sandra; García-Fernández, Alfredo ; Iriondo, Jose M.; Lara-Romero, Carlos
alpine plants
functional annotation
Issue DateOct-2019
PublisherCambridge University Press
CitationPlant Genetic Resources 17(5): 452-455 (2019)
AbstractSilene ciliata (Caryophyllaceae) is a key species to test evolutionary hypotheses in a global warming context. The recent advances in Next Generation Sequencing technologies can help in providing clues about climate-mediated local adaptation. In the present study, we analysed the full transcriptome of six individuals of S. ciliata from Central Spain, by aligning it with the transcriptome of S. latifolia. We aimed (a) to identify Single Nucleotide Polymorphisms (SNPs) in the transcriptome of the species, (b) to describe the biological function of the polymorphic genes expressed and (c) to identify loci that may be involved in local adaptation processes at optimal and marginal populations of the species. We identified a total of 147,118 SNPs distributed throughout 12,688 sequences. The number of polymorphic sequences annotated was 8023. One hundred thirty sequences containing polymorphisms strongly associated with optimal and marginal conditions were selected. Gene ontology searches were successful for 118, and many of these were related to responses to stress (n = 19) and abiotic stimulus (n = 16). Genomic data generated provide a starting point for further research on the identification of candidate genes related to local adaptation and other processes in the species.
Publisher version (URL)http://doi.org/10.1017/S1479262119000157
Identifiersdoi: 10.1017/S1479262119000157
e-issn: 1479-263X
issn: 1479-2621
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