Evaluation of immune response of marine-derived collagen and gelatin

Abstract

136P26EVALUATION OF IMMUNE RESPONSE OF MARINE-DERIVED COLLAGEN AND GELATINA. L. Alves1, 2, J. Costa-Gouveia 2, 3, Carmen G. Sotelo 5, J.A. Vázquez 5, R. I. Pérez-Martín 5, E. Torrado 2, 3, R. L. Reis1,2,4, A.G. Castro 2,3T. H. Silva1, 213B’s Research Group, I3Bs –Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Barco, Guimarães, Portugal; 2ICVS/3B’s –PT Government Associate Laboratory, Braga/Guimarães, Portugal; 3Microbiology and Infection Research Domain, Life and Health Sciences Research Institute, School of Medicine, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal; 4The Discoveries Centre for Regenerative and Precision Medicine, Headquarters at University of Minho, Avepark, 4805-017 Barco, Guimarães, Portugal; 5Instituto de Investigaciones Marinas (IIM-CSIC), C/Eduardo Cabello 6, Vigo, Pontevedra, SpainThe main goal of Tissue Engineering and Regenerative Medicine (TERM) is the attempt to replace and/or regenerate a damaged cells, tissue or organs with a well-designed and tissue appropriated material [1]. This replacement can be performed using a combination of biomaterial, cells and biochemical signals but also using the biomaterial per sethat can stimulate and induce the regeneration of host tissue itself [2]. The successfulness of this relies on the host response to the biomaterial, namely, the reaction of immune cells to the implant. A correct understanding of the immune system and how it interacts with the biomaterial in order to promote the remodelling is 137extremely important to the effective tissue regeneration. Marine-derived collagens and gelatines are promising biopolymers to be used as building blocks for the development of biomaterials in the field of TERM. The absence of any religious constraints and risks associated to zoonosis (in opposition to bovine collagen and the related bovine spongiform encephalopathy, BSE) have demonstrated to be important advantages. However, there is a lack of information in literature about the immunological impact of those materials, existing only a few reports using marine collagen from squid [3], tilapia [4], jellyfish [5]and carp [6]. Given this, our work represents, to our best knowledge, the first specific study of immunological response of different marine origin collagens and gelatines. By-products from Galician and Portuguese fishing industries, particularly blue shark and cod skins were used as raw materials to produce collagen and gelatin. Bone Marrow Derived Macrophages (BMDM) isolated from C57Bl.6 mice were stimulated with the extracted type I collagen and gelatine. At different time points after stimulation, both the cells and supernatant were collected, and cytokine profile assessed. Cytokines qRT-PCR results suggest that our materials induce a M2-like phenotype (recognized as anti-inflammatory and pro-regenerative) of BMDM, indicating that those biopolymers can create a cell-microenvironment that can be advantageous for tissue regeneration