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Título: | A simple and versatile microfluidic device for efficient biomacromolecule crystallization and structural analysis by serial crystallography |
Autor: | De Wijn, Raphäel; Henning, Oliver; Roche, Jennifer; Engilberge, Sylvain; Rollet, Kevin; Fernández Millán, Pablo; Brillet, Karl; Betat, Heike; Mörl, Mario; Roussel, Alain; Girard, Eric; Mueller Dieckmann, Christoph; Fox, Gavin C.; Olieric, Vincent; Gavira Gallardo, J. A. CSIC ORCID ; Lorber, Bernard; Sauter, Claude | Fecha de publicación: | 19-abr-2019 | Editor: | International Union of Crystallography | Citación: | IUCrJ 6: 454- 464 (2019) | Resumen: | Determining optimal conditions for the production of well diffracting crystals is a key step in every biocrystallography project. Here, a microfluidic device is described that enables the production of crystals by counter-diffusion and their direct on-chip analysis by serial crystallography at room temperature. Nine 'non-model' and diverse biomacromolecules, including seven soluble proteins, a membrane protein and an RNA duplex, were crystallized and treated on-chip with a variety of standard techniques including micro-seeding, crystal soaking with ligands and crystal detection by fluorescence. Furthermore, the crystal structures of four proteins and an RNA were determined based on serial data collected on four synchrotron beamlines, demonstrating the general applicability of this multipurpose chip concept. | Versión del editor: | http://dx.doi.org/10.1107/S2052252519003622 | URI: | http://hdl.handle.net/10261/204326 | DOI: | 10.1107/S2052252519003622 | Identificadores: | doi: 10.1107/S2052252519003622 issn: 2052-2525 |
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