English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/20425
Título

Effects on feeding rate and biomarker responses of marine mussels experimentally exposed to propranolol and acetaminophen

AutorSolé, Montserrat ; Shaw, Jennifer P.; Frickers, Patricia E.; Readman, James W.; Hutchinson, Thomas H.
Palabras claveMytilus galloprovincialis
Propranolol
Acetaminophen
Feeding rate
Biochemical responses
Fecha de publicaciónene-2010
EditorSpringer
CitaciónAnalytical and Bioanalytical Chemistry 396(2): 649-656 (2010)
ResumenEnvironmental risk assessments of human pharmaceuticals and other ‘emerging contaminants’ should integrate both population-relevant endpoints and biomarkers of potential modes of action in a range of species. Adult Mytilus galloprovincialis were exposed to the beta-adrenergic receptor blocker propranolol or to the anti-inflammatory drug acetaminophen (paracetamol), both commonly used therapeutic drugs present in aquatic ecosystems. Mussels were exposed under semi-static conditions for 10 days to either acetaminophen (CAS number 103-90-2; mean measured concentrations 23 and 403 µg/L) or propranolol hydrochloride (CAS number 318-98-9; mean measured propranolol concentrations 11 and 147 µg/L) at 15 ± 1 °C sea water. Feeding rate was assessed as an indicator of general toxicity. For propranolol, the 10-day no-observed effect concentration (feeding rateNOEC) and lowest observed effect concentration (feeding rateLOEC) were 11 and 147 µg/L, respectively. For acetaminophen, feeding rate was increased at both 23 and 403 µg/L, suggesting a 10-day feeding rateNOEC of 403 µg/L. Primarily, phase I carboxylesterase (CbE), phase II glutathione S-transferase (GST) and the anti-oxidant catalase activities were evaluated in digestive gland. Gill GST and acetylcholinesterase (AChE) activities were also measured. Lipid peroxidation (LPO) levels were measured in both tissues to assess oxidative stress. Some enzymatic activities in liver were also reduced after propranolol exposure whilst acetaminophen enhanced them (CbE p < 0.05). Acetaminophen exposure significantly increased hepatic LPO levels and inhibited AChE activity in gill (10-day NOEC and LOEC of 23 and 403 µg/L, respectively), whereas propranolol (11 µg/L) enhanced gill GST.
Descripción8 pages, 2 figures
Versión del editorhttp://dx.doi.org/10.1007/s00216-009-3182-1
URIhttp://hdl.handle.net/10261/20425
DOI10.1007/s00216-009-3182-1
ISSN1618-2642(print)
1618-2650(online)
Aparece en las colecciones: (ICM) Artículos
Ficheros en este ítem:
No hay ficheros asociados a este ítem.
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.